Synaptophysin Polyklonaler Antikörper

Synaptophysin Polyklonal Antikörper für IF-P, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IF-P, FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

Publikationen(1)

Kat-Nr. : CL647-17785

Synonyme

SYP, SYN, MRX96

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  IF Staining of mouse pancreas using CL647-17785

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite® Plus 647 Synaptophysin antibody (CL647-17785) at dilution of 1:200.

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  IF Staining of mouse pancreas using CL647-17785

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CoraLite® Plus 647 Synaptophysin antibody (CL647-17785) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using CL647-17785

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 Synaptophysin antibody (CL647-17785) at dilution of 1:1000.

• 

  IF Staining of mouse brain using CL647-17785

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 Synaptophysin antibody (CL647-17785) at dilution of 1:1000.

• 

  FC experiment of PC-12 using CL647-17785

  1X10^6 PC-12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Synaptophysin (CL647-17785) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Maus-Pankreasgewebe, Maushirngewebe
• Erfolgreiche Detektion in FC (Intra): PC-12-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL647-17785 bindet in IF-P, FC (Intra) Synaptophysin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Synaptophysin fusion protein Ag11781
• Vollständiger Name: synaptophysin
• Berechnetes Molekulargewicht: 313 aa, 34 kDa
• Beobachtetes Molekulargewicht: 38-40 kDa
• GenBank-Zugangsnummer: BC064550
• Gene symbol: Synaptophysin
• Gene ID (NCBI): 6855
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)

Publikationen

Species	Application	Title
mouse	IF	Neuron; Metabolic reprogramming through histone lactylation in microglia and macrophages recruits CD8+ T lymphocytes and aggravates spinal cord injury; Authors - Xuhui Ge