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  • Featured Product
  • KD/KO Validated

Syntaxin 6 Polyklonaler Antikörper

Syntaxin 6 Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-10841

Synonyme

Golgi Marker, STX6, syntaxin 6

Galerie der Validierungsdaten

Filter:
  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using CoraLite® Plus 488-conjugated Syntaxin 6 Polyclona (CL488-10841)

    IF Staining of PC-12 using CL488-10841

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using CoraLite® Plus 488 Syntaxin 6 antibody (CL488-10841) at dilution of 1:100.

  • Flow cytometry (FC) experiment of PC-12 cells using CoraLite® Plus 488-conjugated Syntaxin 6 Polyclona (CL488-10841)

    FC experiment of PC-12 using CL488-10841

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Syntaxin 6 (CL488-10841) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFPC-12-Zellen
Erfolgreiche Detektion in FCPC-12-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-10841 bindet in IF, FC (Intra) Syntaxin 6 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Syntaxin 6 fusion protein Ag1284
Vollständiger Name syntaxin 6
Berechnetes Molekulargewicht 29 kDa
Beobachtetes Molekulargewicht 32 kDa
GenBank-ZugangsnummerBC009944
Gene symbol STX6
Gene ID (NCBI) 10228
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 Syntaxin 6 antibody CL488-10841Protokoll herunterladen
FC protocol for CL Plus 488 Syntaxin 6 antibody CL488-10841Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen