TAB1 Polyklonaler Antikörper

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• KD/KO Validated

TAB1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 27566-1-AP

Synonyme

MAP3K7IP1, Mitogen-activated protein kinase kinase kinase 7-interacting protein 1, TAK1 binding protein 1, TAK1-binding protein 1, TGF-beta-activated kinase 1 and MAP3K7-binding protein 1

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  WB analysis using 27566-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27566-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 27566-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 27566-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27566-1-AP (TAB1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 27566-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27566-1-AP (TAB1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 27566-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27566-1-AP (TAB1 antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HCT 116-Zellen, HEK-293T-Zellen, HeLa-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

27566-1-AP bindet in WB, IHC, IF, IP, ELISA TAB1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TAB1 fusion protein Ag25576
• Vollständiger Name: mitogen-activated protein kinase kinase kinase 7 interacting protein 1
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC050554
• Gene symbol: TAB1
• Gene ID (NCBI): 10454
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IP	Blood; Platelet NLRP6 protects against microvascular thrombosis in sepsis; Authors - Huimin Jiang
human	WB	Theranostics; Virus-induced p38 MAPK activation facilitates viral infection.; Authors - Yuting Cheng ; KO Validated
human	WB,IF	Int J Mol Sci; MicroRNA-27a-5p Downregulates Expression of Proinflammatory Cytokines in Lipopolysaccharide-Stimulated Human Dental Pulp Cells via the NF-κB Signaling Pathway; Authors - Shihan Wang ; KD Validated