TAF15 Polyklonaler Antikörper

TAF15 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, RIP, IHC, IF

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 24235-1-AP

Synonyme

hTAFII68, RBP56, RNA binding protein 56, TAF(II)68, TAF15, TAF2N, TAFII68

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 24235-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24235-1-AP (TAF15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 24235-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 24235-1-AP (TAF15 Antibody) at dilution of 1:50.

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  IHC staining of human kidney using 24235-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 24235-1-AP (TAF15 Antibody) at dilution of 1:50.

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  IF Staining of HEK-293 using 24235-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 24235-1-AP (TAF15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• RIP: See 1 publications below

Produktinformation

24235-1-AP bindet in WB, RIP, IHC, IF TAF15 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TAF15 fusion protein Ag20828
• Vollständiger Name: TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
• Berechnetes Molekulargewicht: 592 aa, 62 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC046099
• Gene symbol: TAF15
• Gene ID (NCBI): 8148
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TAF15 (TAF2N or TAFII68) is a nuclear protein known to associate with a distinct subpopulation of transcription factor IID (TFIID), a multi-subunit complex that nucleates the pre-initiation complex on promoters of on protein-coding genes. TAF15 harbors a transcriptional activation domain, a RNA recognition motif and many Arg-Gly-Gly repeats that participate in RNA binding. TAF15 along with FUS and EWS constitutes the FET (FUS/EWS/TAF15) protein family of heterogeneous nuclear ribonucleoproteins (hnRNPs) class of RNA binding proteins that are multifunctional proteins implicated in numerous aspects of RNA processing/functions. TAF15 is a nuclear protein that shuttle between cytoplasm-nucleus and as RNA/ssDNA-binding protein, it play specific roles during transcription initiation at distinct promoters and enter the preinitiation complex together with the RNA polymerase II. TAF15 as well as other FET member genes are frequently translocated in sarcomas and rare hematopoietic as well as epithelial cancers. Moreover, dominant mutations in genes coding for four hnRNPs (TDP-43, FUS/TLS, TAF15, and EWS) are found in familial and sporadic cases of ALS and a chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC).

Publikationen

Species	Application	Title
human	WB,RIP	Front Oncol; Long Non-Coding RNA CCAT2 Activates RAB14 and Acts as an Oncogene in Colorectal Cancer.; Authors - Dalu Wang