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  • KD/KO Validated

WWTR1/TAZ Monoklonaler Antikörper

WWTR1/TAZ Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

CloneNo.

2A12A10

Kat-Nr. : 66500-1-Ig

Synonyme

TAZ, WWTR1, 2A12A10, Transcriptional coactivator with PDZ-binding motif, WW domain-containing transcription regulator protein 1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA549-Zellen, 4T1-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen, MDA-MB-231-Zellen, MDA-MB-453s-Zellen, NIH/3T3-Zellen
Erfolgreiche Detektion in IHChumanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-Phumanes Leberkarzinomgewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunhistochemie (IHC)IHC : 1:300-1:1200
Immunfluoreszenz (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66500-1-Ig bindet in WB, IHC, IF-P, ELISA WWTR1/TAZ und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen WWTR1/TAZ fusion protein Ag13330
Vollständiger Name WW domain containing transcription regulator 1
Berechnetes Molekulargewicht 44 kDa
Beobachtetes Molekulargewicht 55 kDa
GenBank-ZugangsnummerBC014052
Gene symbol WWTR1
Gene ID (NCBI) 25937
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma. TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for WWTR1/TAZ antibody 66500-1-IgProtokoll herunterladen
IHC protocol for WWTR1/TAZ antibody 66500-1-IgProtokoll herunterladenl
IF protocol for WWTR1/TAZ antibody 66500-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Cell Death Differ

Targeting USP10 induces degradation of oncogenic ANLN in esophageal squamous cell carcinoma

Authors - Yu-Fei Cao
humanWB

Nat Commun

LPA signaling acts as a cell-extrinsic mechanism to initiate cilia disassembly and promote neurogenesis.

Authors - Huai-Bin Hu
  • KD Validated
mouseWB,IF

Cell Signal

RhoA/ROCK-TAZ Axis regulates bone formation within calvarial trans-sutural distraction osteogenesis

Authors - Junjie Ye
mouseWB,IHC,IF

Front Pharmacol

TAZ Induces Migration of Microglia and Promotes Neurological Recovery After Spinal Cord Injury.

Authors - Xuyang Hu
humanWB

Am J Transl Res

VASN promotes YAP/TAZ and EMT pathway in thyroid carcinogenesis in vitro.

Authors - Adheesh Bhandari
ratWB,IF

Toxicol Appl Pharmacol

Cysteine and glycine rich protein 2 exacerbates vascular fibrosis in pulmonary hypertension through the nuclear translocation of yes-associated protein and transcriptional coactivator with PDZ-binding motif

Authors - Xinghe Chen