TBL2 Monoklonaler Antikörper

TBL2 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgA

Getestete Reaktivität

human

Anwendung

WB, IHC

Konjugation

Unkonjugiert

CloneNo.

1D5C2

Kat-Nr. : 66307-1-Ig

Synonyme

TBL2, transducin (beta) like 2, Transducin beta like protein 2, WBSCR13, WS betaTRP

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 66307-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66307-1-Ig (TBL2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66307-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66307-1-Ig (TBL2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 66307-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66307-1-Ig (TBL2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 66307-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66307-1-Ig (TBL2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 66307-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66307-1-Ig (TBL2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 66307-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66307-1-Ig (TBL2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 66307-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66307-1-Ig (TBL2 Antibody) at dilution of 1:200 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, COLO 320-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66307-1-Ig bindet in WB, IHC TBL2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgA
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TBL2 fusion protein Ag24225
• Vollständiger Name: transducin (beta)-like 2
• Berechnetes Molekulargewicht: 446 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC012938
• Gene symbol: TBL2
• Gene ID (NCBI): 26608
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Thiophiler Affinitätschromatograph
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TBL2 belongs to a member of the beta-transducin protein family. This gene is commonly deleted in Williams-Beuren syndrome, a developmental disorder with multi-system manifestations caused by haploinsufficiency for contiguous genes deleted in chromosome region 7q11.23. TBL2 encodes a protein with four putative WD40 repeats, which may be involved in some intracellular signaling pathway.