TBP Polyklonaler Antikörper
TBP Polyklonal Antikörper für WB, IP, IHC, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (2)
Anwendung
WB, IHC, IF, IP, ChIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 22006-1-AP
Synonyme
"TBP Antibodies" Comparison
View side-by-side comparison of TBP antibodies from other vendors to find the one that best suits your research needs.
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | COLO 320-Zellen |
Erfolgreiche IP | COLO 320-Zellen |
Erfolgreiche Detektion in IHC | Maushodengewebe, humanes Mammakarzinomgewebe, Rattenlebergewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 79 publications below |
IHC | See 1 publications below |
IF | See 3 publications below |
IP | See 3 publications below |
ChIP | See 1 publications below |
Produktinformation
22006-1-AP bindet in WB, IHC, IF, IP, ChIP, ELISA TBP und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Affe, Hausschwein, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | TBP fusion protein Ag12383 |
Vollständiger Name | TATA box binding protein |
Berechnetes Molekulargewicht | 338 aa, 38 kDa |
Beobachtetes Molekulargewicht | mouse/rat 33-36 kDa and human 37-43kDa |
GenBank-Zugangsnummer | BC110341 |
Gene symbol | TBP |
Gene ID (NCBI) | 6908 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody recognize human TBP with MW 40 kDa and mouse/rat Tbp with MW 34 kDa.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for TBP antibody 22006-1-AP | Protokoll herunterladen |
IHC protocol for TBP antibody 22006-1-AP | Protokoll herunterladenl |
IP protocol for TBP antibody 22006-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Hepatology Steatotic hepatocytes release mature VLDL via methionine and tyrosine metabolism in a Keap1-Nrf2 dependent manner. | ||
Redox Biol Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke | ||
Cancer Res DNA of neutrophil extracellular traps binds TMCO6 to impair CD8+ T-cell immunity in hepatocellular carcinoma | ||
J Hazard Mater ZnO NPs delay the recovery of psoriasis-like skin lesions through promoting nuclear translocation of p-NFκB p65 and cysteine deficiency in keratinocytes. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Mi (Verified Customer) (06-25-2023) | This antibody works very well in human white and brown adipocyte cells.
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