TBR1 Polyklonaler Antikörper

TBR1 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(36)

Rezensionen (2)

Kat-Nr. : 20932-1-AP

Synonyme

TBR-1, TBR 1, T-box brain protein 1, T brain 1, T box, brain, 1

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  WB analysis of mouse brain using 20932-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20932-1-AP (TBR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 20932-1-AP

  IP result of anti-TBR1 (IP:20932-1-AP, 4ug; Detection:20932-1-AP 1:600) with mouse brain tissue lysate 4000ug.

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  IHC staining of human brain using 20932-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20932-1-AP (TBR1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 20932-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20932-1-AP (TBR1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of rat brain using 20932-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20932-1-AP (TBR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 20932-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20932-1-AP (TBR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20932-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20932-1-AP (TBR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20932-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20932-1-AP (TBR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 20932-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TBR1 antibody (20932-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 20932-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TBR1 antibody (20932-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IHC: See 5 publications below
• IF: See 24 publications below

Produktinformation

20932-1-AP bindet in WB, IHC, IF-P, IP, ELISA TBR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TBR1 fusion protein Ag14935
• Vollständiger Name: T-box, brain, 1
• Berechnetes Molekulargewicht: 682 aa, 74 kDa
• Beobachtetes Molekulargewicht: 74 kDa
• GenBank-Zugangsnummer: BC104844
• Gene symbol: TBR1
• Gene ID (NCBI): 10716
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TBR1, also named as T-box brain protein 1, is a 682 amino acid protein, which contains one T-box DNA-binding domain and localizes in the nucleus. TBR1 is expressed in the brain and as a transcriptional regulator is involved in developmental processes. TBR1 is required for normal brain development.

Publikationen

Species	Application	Title
human,mouse	IF	Nat Neurosci; A tau homeostasis signature is linked with the cellular and regional vulnerability of excitatory neurons to tau pathology.; Authors - Hongjun Fu
human	IF	Nat Commun; GRAMD1B is a regulator of lipid homeostasis, autophagic flux and phosphorylated tau; Authors - Diana Acosta Ingram
mouse	IF	Nat Commun; Pathogenic POGZ mutation causes impaired cortical development and reversible autism-like phenotypes.; Authors - Kensuke Matsumura
mouse	IF	Nat Commun; Disrupted neuronal maturation in Angelman syndrome-derived induced pluripotent stem cells.; Authors - James J Fink
mouse	IHC	Mol Neurodegener; AIF3 splicing switch triggers neurodegeneration.; Authors - Shuiqiao Liu
human	IHC	Proc Natl Acad Sci U S A; Human intermediate progenitor diversity during cortical development.; Authors - Mark-Phillip Pebworth

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (03-01-2024)

Used on human brain cortical sections, it marked nicely the nucleus of my neurons.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

TBR1 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:20932-1-AP)

FH

Mandi (Verified Customer) (03-09-2020)

Good staining on iPSC-derived Cortical neurons. Needed a decent amount of optimization to prevent non-specific binding though. Make sure to run primary/secondary deletes.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: human cortical neurons