TBX18 Polyklonaler Antikörper

TBX18 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 23237-1-AP

Synonyme

T box 18, T box protein 18, TBX18

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 23237-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23237-1-AP (TBX18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23237-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23237-1-AP (TBX18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 23237-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 23237-1-AP (TBX18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 23237-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 23237-1-AP (TBX18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23237-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23237-1-AP (TBX18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 23237-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23237-1-AP (TBX18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 23237-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23237-1-AP (TBX18 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 23237-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23237-1-AP (TBX18 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 23237-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23237-1-AP (TBX18 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 23237-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23237-1-AP (TBX18 Antibody) at dilution of 1:50 (under 10x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, humanes Plazenta-Gewebe, Mausnierengewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

23237-1-AP bindet in WB, IHC, IF, ELISA TBX18 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TBX18 fusion protein Ag19258
• Vollständiger Name: T-box 18
• Berechnetes Molekulargewicht: 607 aa, 65 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC157841
• Gene symbol: TBX18
• Gene ID (NCBI): 9096
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The T-box (Tbx) motif is present in a family of genes whose structural features and expression patterns support their involvement in developmental gene regulation. The Tbx gene family are largely conserved throughout metazoan evolution, and these genes code for putative transcription factors that share a uniquely defining DNA-binding domain. Tbx genes are a family of developmental regulators with more than 20 members recently identified in invertebrates and vertebrates. Mutations in Tbx genes are associated with the onset of several human diseases. Our understanding of functional mechanisms of Tbx products has come mainly from the prototypical T/Brachyury, which is a transcription activator. The Tbx genes constitute a family of transcriptional regulatory genes that are implicated in a variety of developmental processes ranging from the formation of germ layers to the organizational patterning of the central nervous system.This antibody specifically reacts with the 65kd TBX18 protein.

Publikationen

Species	Application	Title
human	IF	EBioMedicine; E2A ablation enhances proportion of nodal-like cardiomyocytes in cardiac-specific differentiation of human embryonic stem cells.; Authors - Xiuya Li
rat	WB	Mol Med Rep; G9a inhibition promotes the formation of pacemaker-like cells by reducing the enrichment of H3K9me2 in the HCN4 promoter region; Authors - Pei Xu