TBXAS1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

TBXAS1 Monoklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1F6G6

Kat-Nr. : 67371-1-Ig

Synonyme

Hydroperoxy icosatetraenoate dehydratase, EC:5.3.99.5, EC:4.2.1.152, CYP5A1, CYP5

---

• 

  WB analysis of human peripheral blood platelets using 67371-1-Ig

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67371-1-Ig (TBXAS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 67371-1-Ig

  WB result of TBXAS1 antibody (67371-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBXAS1 transfected A549 cells.

• 

  WB analysis of A549 using 67371-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67371-1-Ig (TBXAS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig blood using 67371-1-Ig

  pig blood were subjected to SDS PAGE followed by western blot with 67371-1-Ig (TBXAS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 67371-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67371-1-Ig (TBXAS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 67371-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67371-1-Ig (TBXAS1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 67371-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67371-1-Ig (TBXAS1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of A549 using 67371-1-Ig

  1x10^6 A549 cells were intracellularly stained with 0.8 ug TBXAS1 Monoclonal antibody (67371-1-Ig, Clone:1F6G6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human peripheral blood platelets, A549-Zellen, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

67371-1-Ig bindet in WB, IHC, FC (Intra), ELISA TBXAS1 und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TBXAS1 fusion protein Ag29177
• Vollständiger Name: thromboxane A synthase 1 (platelet)
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC014117
• Gene symbol: TBXAS1
• Gene ID (NCBI): 6916
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TBXAS1 catalyzes the conversion of the prostaglandin endoperoxide into thromboxane A2 as a potent vasoconstrictor and inducer of platelet aggregation.It was identified as novel airway fibroblast-specific markers with integrin-8 (ITGA8) (PMID:20435685). It was observed in the epithelium.It is a monomer with the molecular weight of 60 KDa. It also has two variants with 60.5 kDa and 52.3 kDa (PMID:1730669). It also reavels a molecular weight of 55 kDa in human uterus (PMID:1386258).

Publikationen

Species	Application	Title
mouse	WB,IHC	Am J Physiol Renal Physiol; Inhibition of COX-1 attenuates the formation of thromboxane A2 and ameliorates the acute decrease in glomerular filtration rate in endotoxemic mice.; Authors - Katharina Mederle