TERF1 Monoklonaler Antikörper

TERF1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1D10G4

Rezensionen (1)

Kat-Nr. : 67592-1-Ig

Synonyme

Telomeric repeat-binding factor 1, t TRF1, PIN2, NIMA-interacting protein 2, hTRF1 AS

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• 

  WB analysis of K-562 using 67592-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67592-1-Ig (TERF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67592-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67592-1-Ig (TERF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67592-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67592-1-Ig (TERF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HEK-293-Zellen, Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

67592-1-Ig bindet in WB, IP, ChIP, ELISA TERF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TERF1 fusion protein Ag12540
• Vollständiger Name: telomeric repeat binding factor (NIMA-interacting) 1
• Berechnetes Molekulargewicht: 439 aa, 50 kDa
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: BC029378
• Gene symbol: TERF1
• Gene ID (NCBI): 7013
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Telomeres are nucleoprotein complexes located at chromosome ends that consist of tandem arrays of TTAGGG repeats bound to specific proteins. Telomeres protect chromosome ends from degradation and end-to-end fusions, prevent activation of DNA damage checkpoints, and modulate the maintenance of telomeric DNA by telomerase. TERF1 (telomeric repeat-binding factor 1) binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. It's one component of the shelterin complex, which involved in the regulation of telomere length and protection.

Publikationen

Species	Application	Title
human	WB,IP,ChIP	Nat Commun; Transcription stress at telomeres leads to cytosolic DNA release and paracrine senescence; Authors - Athanasios Siametis

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Refik (Verified Customer) (08-22-2025)

Antibody works really well at a 1:250 - 500 dilution for ICC imaging of 2D and 3D cardiac cells with secondary Alexa Flour antibody at 1:400 - 500 and DAPI for both human and mouse specific cells. Works really well at 1:1000 concentration in western blot for overnight incubation followed by 1:300 secondary antibody incubation for 1 hour for human and mouse tissue.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:250 for ICC and 1:1000 for WB
• Cell Tissue Type: Cardiac myocytes human and mouse derived