TET2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TET2 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

57

Rezensionen (2)

Kat-Nr. : 21207-1-AP

Synonyme

KIAA1546, tet oncogene family member 2, TET2

---

Galerie der Validierungsdaten

• 

  WB analysis using 21207-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21207-1-AP (TET2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 21207-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 21207-1-AP (TET2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 21207-1-AP

  IP Result of anti-TET2 (IP:21207-1-AP, 3ug; Detection:21207-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human colon cancer using 21207-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21207-1-AP (TET2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 21207-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21207-1-AP (TET2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Neuro-2a using 21207-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using 21207-1-AP (TET2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 21207-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TET2 (21207-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, HL-60-Zellen, K-562-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Neuro-2a-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 12 publications below
• WB: See 47 publications below
• IHC: See 9 publications below
• IF: See 12 publications below
• CoIP: See 2 publications below
• ChIP: See 3 publications below

Produktinformation

21207-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA TET2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TET2 fusion protein Ag15583
• Vollständiger Name: tet oncogene family member 2
• Berechnetes Molekulargewicht: 2002 aa, 224 kDa
• Beobachtetes Molekulargewicht: 130 kDa, 250 kDa
• GenBank-Zugangsnummer: BC150180
• Gene symbol: TET2
• Gene ID (NCBI): 54790
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TET2 is an enzyme which converts 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC) in DNA and is important for normal myelopoiesis. TET2 mutations are frequently observed in myeloid malignancies, including myeloproliferative neoplasms. TET2 is broadly expressed in a variety of tissues. In healthy tissues, TET2 expression was shown to be elevated in hematopoietic cells with highest expression in granulocytes. Three isoforms of TET2 exists due to the alternative splicing. 220-250 kDa bands represent the full-length isoform; 130-133 kDa bands represent the nuclear isoforms of TET2. (PMID: 24615693) This antibody recognizes all three isoforms of TET2.

Publikationen

Species	Application	Title
human	WB	Adv Sci (Weinh); m6A-Modified circTET2 Interacting with HNRNPC Regulates Fatty Acid Oxidation to Promote the Proliferation of Chronic Lymphocytic Leukemia; Authors - Zijuan Wu
human	WB	Sci Adv; The chromosomal protein SMCHD1 regulates DNA methylation and the 2c-like state of embryonic stem cells by antagonizing TET proteins.; Authors - Zhijun Huang
mouse	CoIP	Sci Adv; Reprogramming of DNA methylation at NEUROD2-bound sequences during cortical neuron differentiation.; Authors - Maria A Hahn
mouse	WB	Nat Commun; Redirected nuclear glutamate dehydrogenase supplies Tet3 with α-ketoglutarate in neurons.; Authors - Franziska R Traube
mouse	WB	J Exp Med; TGF-β signaling controls Foxp3 methylation and T reg cell differentiation by modulating Uhrf1 activity.; Authors - Xiang Sun
mouse	WB	JACC Basic Transl Sci; Hypermethylation of the Micro-RNA 145 Promoter Is the Key Regulator for NLRP3 Inflammasome-Induced Activation and Plaque Formation.; Authors - Wei Zhong

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Melanie (Verified Customer) (11-19-2019)

IHC of 5um thin skin sections was performed. A positive staining was observed in the skin epithelial layer as predicted by the human protein atlas. Citrate buffer pH6 was used for antigen retrieval

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: Skin

TET2 Antibody Immunohistochemistry, validation (1:400 dilution) in Skin (Cat no:21207-1-AP)

FH

Sid (Verified Customer) (04-12-2019)

Product performed well in western blot

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: K562

Verwandte Produkte

EZH2 Polyclonal antibody

21800-1-AP

JAK2 Polyclonal antibody

17670-1-AP

IDH1 Polyclonal antibody

12332-1-AP

IDH1 Monoclonal antibody

66197-1-Ig

IDH2 Polyclonal antibody

15932-1-AP