THUMPD3 Polyklonaler Antikörper

THUMPD3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19807-1-AP

Synonyme

EC:2.1.1.256, THUMP domain containing 3, THUMP domain-containing protein 3, tRNA (guanine(6)-N2)-methyltransferase THUMP3

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  WB analysis using 19807-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19807-1-AP (THUMPD3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19807-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19807-1-AP (THUMPD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 19807-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19807-1-AP (THUMPD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 19807-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19807-1-AP (THUMPD3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocirrhosis using 19807-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19807-1-AP (THUMPD3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 19807-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19807-1-AP (THUMPD3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 19807-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19807-1-AP (THUMPD3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 19807-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19807-1-AP (THUMPD3 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 19807-1-AP

  Immunofluorescent analysis of Hela cells, using THUMPD3 antibody 19807-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 19807-1-AP

  Immunofluorescent analysis of HepG2 cells, using THUMPD3 antibody 19807-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, L02-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19807-1-AP bindet in WB, IHC, IF/ICC, ELISA THUMPD3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: THUMPD3 fusion protein Ag13833
• Vollständiger Name: THUMP domain containing 3
• Berechnetes Molekulargewicht: 507 aa, 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC001622
• Gene symbol: THUMPD3
• Gene ID (NCBI): 25917
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

THUMPD3, or THUMP domain containing 3, is a protein-coding gene that plays a significant role in tRNA methylation, a post-transcriptional modification that affects tRNA biology and is closely associated with human diseases. THUMPD3 is responsible for catalyzing the formation of N2-methylguanosine (m2G) at position 6 in a broad range of tRNA substrates containing the characteristic 3'-CCA terminus of mature tRNAs.