TIMM17B Polyklonaler Antikörper

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• KD/KO Validated

TIMM17B Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Rezensionen (1)

Kat-Nr. : 11062-1-AP

Synonyme

DXS9822, JM3, Mitochondrial import inner membrane translocase subunit Tim17-B, TIM17B

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• 

  WB analysis of PC-3 using 11062-1-AP

  WB result of TIMM17B antibody (11062-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TIMM17B transfected PC-3 cells.

• 

  WB analysis of mouse heart using 11062-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11062-1-AP (TIMM17B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 11062-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11062-1-AP (TIMM17B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 11062-1-AP

  IP result of anti-TIMM17B (IP:11062-1-AP, 4ug; Detection:11062-1-AP 1:1000) with PC-3 cells lysate 1575 ug.

• 

  IHC staining of human colon cancer using 11062-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11062-1-AP (TIMM17B antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Mauslebergewebe, PC-3-Zellen
• Erfolgreiche IP: PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 1 publications below

Produktinformation

11062-1-AP bindet in WB, IHC, IP, ELISA TIMM17B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TIMM17B fusion protein Ag1504
• Vollständiger Name: translocase of inner mitochondrial membrane 17 homolog B (yeast)
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 17-20 kDa
• GenBank-Zugangsnummer: BC010142
• Gene symbol: TIMM17B
• Gene ID (NCBI): 10245
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; Acylglycerol Kinase Maintains Metabolic State and Immune Responses of CD8+ T Cells.; Authors - Zhilin Hu
human	WB	Mol Cell; Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria.; Authors - Milena Vukotic
human	WB	Mol Cell; Sengers Syndrome-Associated Mitochondrial Acylglycerol Kinase Is a Subunit of the Human TIM22 Protein Import Complex.; Authors - Yilin Kang
human		Cell Rep; TIM23 facilitates PINK1 activation by safeguarding against OMA1-mediated degradation in damaged mitochondria; Authors - Shiori Akabane
		Sci Rep; The presence of disulfide bonds reveals an evolutionarily conserved mechanism involved in mitochondrial protein translocase assembly.; Authors - Lidia Wrobel
human	WB	Infect Immun; The type III system-secreted effector EspZ localizes to host mitochondria and interacts with the translocase of inner mitochondrial membrane 17b.; Authors - Shames Stephanie R SR

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Angela (Verified Customer) (12-27-2024)

good

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: heart tissue

TIMM17B Antibody Western Blot validation (1:1000 dilution) in heart tissue (Cat no:11062-1-AP)