TIMP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TIMP1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(100)

Kat-Nr. : 16644-1-AP

Synonyme

TIMP-1, CLGI, Collagenase inhibitor, EPA, Erythroid-potentiating activity

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  WB analysis using 16644-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16644-1-AP (TIMP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16644-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16644-1-AP (TIMP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16644-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16644-1-AP (TIMP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of HT-29 using 16644-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using TIMP1 antibody (16644-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, HeLa-Zellen, HT-29-Zellen, SKOV-3-Zellen
• Erfolgreiche Detektion in IF/ICC: HT-29-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 81 publications below
• IHC: See 25 publications below
• IF: See 7 publications below

Produktinformation

16644-1-AP bindet in WB, IHC, IF/ICC, ELISA TIMP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Kaninchen, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TIMP1 fusion protein Ag10006
• Vollständiger Name: TIMP metallopeptidase inhibitor 1
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC000866
• Gene symbol: TIMP1
• Gene ID (NCBI): 7076
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TIMP1 is a member of the family of matrix metalloproteinase inhibitors, which contains four members (TIMP1, TIMP2, TIMP3, and TIMP4). Tissue inhibitors of metalloproteinases (TIMPs) are multifaceted molecules that exhibit properties beyond their classical proteinase inhibitory function. TIMP1 has several MMP-independent functions such as modulation of angiogenesis, promotion of cell proliferation, and inhibition of apoptosis. TIMP1 plays important role in cell cycle regulation and cancer progression. Recently, clinical studies have shown that the aberrant expression of TIMP1 is associated with an unfavorable prognosis in a series of tumors, such as gastric cancer, papillary thyroid carcinoma, cutaneous melanoma and breast cancer. In pregnancy, TIMP1 plays a regulatory role in the process of implantation, particularly the cytotrophoblast invasion of the uterine endometrium. In pregnancy, TIMP1 plays a regulatory role in the process of implantation, particularly the cytotrophoblast invasion of the uterine endometrium.

Publikationen

Species	Application	Title
	WB	Acta Pharm Sin B; TIMP1 preserves the blood-brain barrier through interacting with CD63/integrin β 1 complex and regulating downstream FAK/RhoA signaling.; Authors - Jingshu Tang
human	WB	Proc Natl Acad Sci U S A; Addiction to Golgi-resident PI4P synthesis in chromosome 1q21.3-amplified lung adenocarcinoma cells.; Authors - Lei Shi
human	WB,IF	J Neuroinflammation; Identification of TIMP1 as an inflammatory biomarker associated with temporal lobe epilepsy based on integrated bioinformatics and experimental analyses; Authors - Ya He
human	WB	Cell Death Dis; α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation; Authors - Hao Wang
human	WB	Biomed Pharmacother; TrxR/Trx inhibitor butaselen ameliorates pulmonary fibrosis by suppressing NF-κB/TGF-β1/Smads signaling; Authors - Yifan Chen
human	IF	Cell Mol Gastroenterol Hepatol; Sestrin Proteins Protect Against Lipotoxicity-Induced Oxidative Stress in the Liver via Suppression of C-Jun N-Terminal Kinases.; Authors - Zhigang Fang