TLK2 Polyklonaler Antikörper

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• KD/KO Validated

TLK2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (1)

Kat-Nr. : 13979-1-AP

Synonyme

PKU ALPHA, TLK2, tousled like kinase 2

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 13979-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13979-1-AP (TLK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13979-1-AP

  WB result of TLK2 antibody (13979-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-TLK2 transfected HeLa cells.

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  WB analysis of HeLa using 13979-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13979-1-AP (TLK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 13979-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13979-1-AP (TLK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13979-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13979-1-AP (TLK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 13979-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13979-1-AP (TLK2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

13979-1-AP bindet in WB, IHC, IF, ELISA TLK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TLK2 fusion protein Ag5022
• Vollständiger Name: tousled-like kinase 2
• Berechnetes Molekulargewicht: 772 aa, 88 kDa
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC044925
• Gene symbol: TLK2
• Gene ID (NCBI): 11011
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tousled-like kinases (TLKs) are a family of serine/threonine kinases located in the nucleus that promote S-phase chromatin assembly and mitotic chromosome segregation. There are two genes in the TLK family-TLK1 and TLK2 (PMID: 14561777). Mutation in TLK2 are associated with an elevated risk of breast cancer (PMID: 27489360). Tousled-like kinase 2 (TLK2) is a key factor in glioblastoma that modulates SRC signaling, thereby driving tumor malignancy. TLK2 is commonly upregulated in glioblastoma, and such upregulation was associated with poor patient outcomes. TLK2 overexpression induced cell growth, migration, invasion, and epithelialmesenchymal transition, and cell cycle arrest, while TLK2 knockdown had the opposite effect (PMID: 30207834). It can detect 3 isoforms of 88, 85 and 82 kDa.

Publikationen

Species	Application	Title
human	WB	Cancer Gene Ther; Targeting TLK2 inhibits the progression of gastric cancer by reprogramming amino acid metabolism through the mTOR/ASNS axis; Authors - Mingliang Wang ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sun (Verified Customer) (01-05-2023)

A selective and sensitiv antibody. KD validated

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T and HCT116