Talin-1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Talin-1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(31)

Rezensionen (2)

Kat-Nr. : 14168-1-AP

Synonyme

ILWEQ, KIAA1027, talin 1, talin1, TLN

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  WB analysis using 14168-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14168-1-AP (Talin-1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14168-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14168-1-AP (Talin-1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14168-1-AP

  IP result of anti-Talin-1 (IP:14168-1-AP, 4ug; Detection:14168-1-AP 1:40000) with HeLa cells lysate 1320 ug.

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  IHC staining of human liver cancer using 14168-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14168-1-AP (Talin-1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14168-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14168-1-AP (Talin-1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 14168-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14168-1-AP (Talin-1 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HUVEC using 14168-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using Talin-1 antibody (14168-1-AP) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of A549 using 14168-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (14168-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• 

  IF Staining of A549 using 14168-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (14168-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 14168-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14168-1-AP (Talin-1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 14168-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14168-1-AP (Talin-1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The cytoskeleton was labelled in red with 66031-1-Ig (alpha tubulin).

• 

  IF Staining of HepG2 using 14168-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14168-1-AP (Talin-1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HUVEC using 14168-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using Talin-1 antibody (14168-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 14168-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug Talin-1 Polyclonal antibody (14168-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, C6-Zellen, HeLa-Zellen, HepG2-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HUVEC-Zellen, A549-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:150-1:600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 22 publications below
• IHC: See 4 publications below
• IF: See 14 publications below
• IP: See 1 publications below

Produktinformation

14168-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA Talin-1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Talin-1 fusion protein Ag5367
• Vollständiger Name: talin 1
• Berechnetes Molekulargewicht: 2541 aa, 270 kDa
• Beobachtetes Molekulargewicht: 250-270 kDa
• GenBank-Zugangsnummer: BC042923
• Gene symbol: Talin-1
• Gene ID (NCBI): 7094
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).

Publikationen

Species	Application	Title
human	IF	ACS Nano; Nanotopography Enhances Dynamic Remodeling of Tight Junction Proteins through Cytosolic Liquid Complexes.; Authors - Xiao Huang
human	WB	Antiviral Res; MβCD inhibits SFTSV entry by disrupting lipid raft structure of the host cells; Authors - Min Cheng
human	WB,IP,IHC,IF	Elife; Binding blockade between TLN1 and integrin β1 represses triple-negative breast cancer.; Authors - Yixiao Zhang ; KD Validated
human	IF	Mol Oncol; ITIH5 as a multifaceted player in pancreatic cancer suppression, impairing tyrosine kinase signaling, cell adhesion and migration; Authors - Jennifer Kosinski
chicken	WB	Microbiol Spectr; Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.; Authors - Beibei Zhang ; KD Validated
mouse	IF	Mol Neurobiol; Essential Role of Sptan1 in Cochlear Hair Cell Morphology and Function Via Focal Adhesion Signaling.; Authors - Qingxiu Yao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (08-12-2025)

Worked well for both WB and IF

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: WB 1:2000, IF, 1:300
• Cell Tissue Type: MSCs

FH

Elham (Verified Customer) (05-09-2025)

Really good staining. Used 1:200 dilution overnight at 4C and stained using Alexa Fluor 488 and actin Cell Mask 647

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Human Airway Smooth Muscle Cell

Talin-1 Antibody Immunofluorescence validation (1:200 dilution) in Human Airway Smooth Muscle Cell (Cat no:14168-1-AP)