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TLR2 Monoklonaler Antikörper
TLR2 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA
Wirt / Isotyp
Maus / IgG2a
Getestete Reaktivität
human und mehr (1)
Anwendung
WB, IHC, IF/ICC, ELISA
Konjugation
Unkonjugiert
CloneNo.
1C1D3
Kat-Nr. : 66645-1-Ig
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | THP-1-Zellen, U-937-Zellen | 
| Erfolgreiche Detektion in IHC | humanes Lebergewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. | 
| Erfolgreiche Detektion in IF/ICC | THP-1-Zellen | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 | 
| Immunhistochemie (IHC) | IHC : 1:200-1:800 | 
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 1 publications below | 
| WB | See 26 publications below | 
| IHC | See 1 publications below | 
| IF | See 4 publications below | 
Produktinformation
66645-1-Ig bindet in WB, IHC, IF/ICC, ELISA TLR2 und zeigt Reaktivität mit human
| Getestete Reaktivität | human | 
| In Publikationen genannte Reaktivität | human, Maus | 
| Wirt / Isotyp | Maus / IgG2a | 
| Klonalität | Monoklonal | 
| Typ | Antikörper | 
| Immunogen | TLR2 fusion protein Ag25626 | 
| Vollständiger Name | toll-like receptor 2 | 
| Berechnetes Molekulargewicht | 784 aa, 90 kDa | 
| Beobachtetes Molekulargewicht | 90-100 kDa | 
| GenBank-Zugangsnummer | BC033756 | 
| Gene symbol | TLR2 | 
| Gene ID (NCBI) | 7097 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Protein-A-Reinigung | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
TLR2, also known as CD282, is a Toll-like receptor (TLR) family member. TLR receptors are type I integral transmembrane glycoproteins that play a fundamental role in pathogen recognition and activation of innate immunity. TLR2 is expressed by immune cells including monocytes/macrophages, polymorphonuclear leukocytes (PMLs), and dendritic cells, and is also expressed by some no-immune cells including endothelial and epithelial cells (PMID: 21428911; 22566960). TLR2 forms heterodimers with TLR1 and TLR6, which is the initial step in a cascade of events leading to significant innate immune responses, development of adaptive immunity to pathogens, and protection from immune sequelae related to infection with these pathogens (PMID: 22566960).
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for TLR2 antibody 66645-1-Ig | Protokoll herunterladen | 
| IHC protocol for TLR2 antibody 66645-1-Ig | Protokoll herunterladenl | 
| IF protocol for TLR2 antibody 66645-1-Ig | Protokoll herunterladen | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| Brain Behav Immun EZH2-mediated suppression of TIMP1 in spinal GABAergic interneurons drives microglial activation via MMP-9-TLR2/4-NLRP3 signaling in neuropathic pain | ||
| Clin Transl Med Monocyte biomarkers define sargramostim treatment outcomes for Parkinson's disease. | ||
| Life Sci The novel mechanism of human norovirus induced diarrhea: Activation of PKD2 caused by HuNoVs destroyed AQP3 expression through AP2γ in intestinal epithelial cells | ||
| Microbes Infect Corydalis Saxicola Bunting Total Alkaloid Eliminates Porphyromonas gingivalis strain 33277 Internalized into Macrophages by Inhibition of TLR2 | ||
| Front Microbiol PPARγ Ameliorates Mycobacterium tuberculosis H37Ra-Induced Foamy Macrophage Formation via the ABCG1-Dependent Cholesterol Efflux Pathway in THP-1 Macrophages. | ||
| ACS Omega Azilsartan Suppressed LPS-Induced Inflammation in U937 Macrophages through Suppressing Oxidative Stress and Inhibiting the TLR2/MyD88 Signal Pathway. | 
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Carly (Verified Customer) (09-27-2019) | Used in an antibody array to identify proteins in human EDTA plasma. 
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