TLR3/CD283 Rekombinanter Antikörper

TLR3/CD283 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

230445G9

Kat-Nr. : CL594-98016-2

Synonyme

TLR3, Toll-like receptor 3, toll like receptor 3, CD283, 230445G9

---

• 

  FC experiment of human monocyte-derived immature dendritic cells using CL594-98016-2

  1x10^6 human monocyte-derived immature dendritic cells were intracellularly stained with 5 ul CoraLite®594 Anti-Human TLR3/CD283 Rabbit Recombinant Antibody (CL594-98016-2, Clone:230445G9) (right), or Isotype Control (left). Cells were co-stained with APC Anti-Human CD11c. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of human monocyte-derived immature dendritic cells using CL594-98016-2

  1x10^6 human monocyte-derived immature dendritic cells were intracellularly stained with 5 ul CoraLite®594 Anti-Human TLR3/CD283 Rabbit Recombinant Antibody (CL594-98016-2, Clone: 230445G9) (red), or Isotype Control (blue). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): human monocyte-derived immature dendritic cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-98016-2 bindet in FC (Intra) TLR3/CD283 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TLR3/CD283 fusion protein Ag34044
• Vollständiger Name: toll-like receptor 3
• Berechnetes Molekulargewicht: 904 aa, 104 kDa
• GenBank-Zugangsnummer: BC094737
• Gene symbol: TLR3
• Gene ID (NCBI): 7098
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

TLR3 belongs to the Toll-like receptor family which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR3 is a nucleotide-sensing TLR that is activated by double-stranded RNA. Upon recognition of dsRNA, TLR3 transmits signals via the adaptor protein TICAM-1/TRIF, leading to the induction of type I IFN, cytokine/chemokine production, and dendritic cell (DC) maturation (PMID: 18262679).