TLR5 Polyklonaler Antikörper

TLR5 Polyklonal Antikörper für IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 19810-1-AP

Synonyme

SLEB1, TIL3, TLR5, toll like receptor 5

---

Galerie der Validierungsdaten

• 

  IP experiment of HeLa using 19810-1-AP

  IP Result of anti-TLR5 (IP:19810-1-AP, 4ug; Detection:19810-1-AP 1:300) with HeLa cells lysate 4000ug.

• 

  IHC staining of human small intestine using 19810-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 19810-1-AP (TLR5 antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human small intestine using 19810-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 19810-1-AP (TLR5 antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human small intestine using 19810-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 19810-1-AP (TLR5 antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human tonsillitis using 19810-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19810-1-AP (TLR5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 19810-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19810-1-AP (TLR5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 19810-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 19810-1-AP (TLR5 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 19810-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 19810-1-AP (TLR5 Antibody) at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe, humanes Eierstockgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 4 publications below
• FC: See 1 publications below

Produktinformation

19810-1-AP bindet in WB, IP, IHC, IF, FC, ELISA TLR5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TLR5 fusion protein Ag13840
• Vollständiger Name: toll-like receptor 5
• Berechnetes Molekulargewicht: 858 aa, 98 kDa
• Beobachtetes Molekulargewicht: 97 kDa
• GenBank-Zugangsnummer: BC109119
• Gene symbol: TLR5
• Gene ID (NCBI): 7100
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TLR5 belongs to the Toll-like receptor family which are important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR5 is known to recognize bacterial flagellin from invading mobile bacteria. TLR5 acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Mutations in TLR5 gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.

Publikationen

Species	Application	Title
mouse	WB	Theranostics; Prussian blue nanozyme-mediated nanoscavenger ameliorates acute pancreatitis via inhibiting TLRs/NF-κB signaling pathway.; Authors - Xue Xie
mouse	FC	Int J Mol Sci; Myeloid-Derived Suppressor Cells Gain Suppressive Function during Neonatal Bacterial Sepsis.; Authors - Jordan K Vance
rat	IHC, IF	Int J Mol Sci; Cross-Talk of Toll-Like Receptor 5 and Mu-Opioid Receptor Attenuates Chronic Constriction Injury-Induced Mechanical Hyperalgesia through a Protein Kinase C Alpha-Dependent Signaling.; Authors - Ching Chang
human	IHC	EBioMedicine; Roseburia intestinalis stimulates TLR5-dependent intestinal immunity against Crohn's disease; Authors - Zhaohua Shen
human	IHC	Oncotarget; Toll-like receptor 5 gene polymorphism is associated with breast cancer susceptibility.; Authors - Chen Shuang
human	WB	Cells; Fibronectin Functions as a Selective Agonist for Distinct Toll-like Receptors in Triple-Negative Breast Cancer.; Authors - Anthony Ambesi

Verwandte Produkte

TLR4 Monoclonal antibody

66350-1-Ig

TLR4 Polyclonal antibody

19811-1-AP

TLR1 Polyclonal antibody

19816-1-AP

TLR3 Polyclonal antibody

17766-1-AP

TLR6 Polyclonal antibody

22240-1-AP

TLR7 Polyclonal antibody

17232-1-AP

NF-κB p65 Polyclonal antibody

10745-1-AP