TLR5 Monoklonaler Antikörper

TLR5 Monoklonal Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

4B3D2

Kat-Nr. : 66570-1-Ig

Synonyme

Toll-like receptor 5, Toll/interleukin-1 receptor-like protein 3, toll like receptor 5, TIL3, SLEB1

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  WB analysis of A549 using 66570-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66570-1-Ig (TLR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of THP-1 using 66570-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66570-1-Ig (TLR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66570-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66570-1-Ig (TLR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 66570-1-Ig

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 66570-1-Ig (TLR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66570-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66570-1-Ig (TLR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of THP-1 using 66570-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TLR5 antibody (66570-1-Ig, Clone: 4B3D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of THP-1 using 66570-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TLR5 antibody (66570-1-Ig, Clone: 4B3D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of THP-1 using 66570-1-Ig

  1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human TLR5 (66570-1-Ig, Clone:4B3D2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, Rattenmilzgewebe, RAW 264.7-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IF/ICC: THP-1-Zellen
• Erfolgreiche Detektion in FC (Intra): THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66570-1-Ig bindet in WB, IF/ICC, FC (Intra), ELISA TLR5 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TLR5 fusion protein Ag13840
• Vollständiger Name: toll-like receptor 5
• Berechnetes Molekulargewicht: 858 aa, 98 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC109119
• Gene symbol: TLR5
• Gene ID (NCBI): 7100
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TLR5 belongs to the Toll-like receptor family which are important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR5 is known to recognize bacterial flagellin from invading mobile bacteria. TLR5 acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Mutations in TLR5 gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.

Publikationen

Species	Application	Title
mouse	WB	Nutr Diabetes; Gestational diabetes exacerbates intrauterine microbial exposure induced intestinal microbiota change in offspring contributing to increased immune response; Authors - Juncheng Liu