TMED4 Polyklonaler Antikörper

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• KD/KO Validated

TMED4 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 14141-1-AP

Synonyme

ERS25, HNLF, TMED4

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 14141-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14141-1-AP (TMED4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14141-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14141-1-AP (TMED4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14141-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14141-1-AP (TMED4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14141-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14141-1-AP (TMED4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 14141-1-AP

  IP result of anti-TMED4 (IP:14141-1-AP, 4ug; Detection:14141-1-AP 1:700) with mouse liver tissue lysate 6000 ug.

• 

  IF Staining of HepG2 using 14141-1-AP

  Immunofluorescent analysis of HepG2 cells using 14141-1-AP (TMED4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HepG2-Zellen
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below

Produktinformation

14141-1-AP bindet in WB, IP, IF, ELISA TMED4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMED4 fusion protein Ag5234
• Vollständiger Name: transmembrane emp24 protein transport domain containing 4
• Berechnetes Molekulargewicht: 227 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC057851
• Gene symbol: TMED4
• Gene ID (NCBI): 222068
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell Res; A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.; Authors - Shulin Li ; KD Validated
human	WB	Ann Transl Med; Screening of the novel immune-suppressive biomarkers of TMED family and whether knockdown of TMED2/3/4/9 inhibits cell migration and invasion in breast cancer; Authors - Zheng Fang ; KD Validated