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TMEM106B (150-274aa) Polyklonaler Antikörper

TMEM106B (150-274aa) Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 20995-1-AP

Synonyme

TMEM106B, Transmembrane protein 106B



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA549-Zellen, HeLa-Zellen, HepG2-Zellen
Erfolgreiche Detektion in IHCRattenhirngewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 6 publications below
IFSee 5 publications below

Produktinformation

20995-1-AP bindet in WB, IHC, IF, ELISA TMEM106B (150-274aa) und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TMEM106B (150-274aa) fusion protein Ag14264
Vollständiger Name transmembrane protein 106B
Berechnetes Molekulargewicht 31 kDa
Beobachtetes Molekulargewicht 31-35 kDa, 40-55 kDa
GenBank-ZugangsnummerBC033901
Gene symbol TMEM106B
Gene ID (NCBI) 54664
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMEM106B is a genetic risk factor for frontotemporal lobar degeneration with TDP-43 inclusions (FTLD-TDP). Amyotrophic lateral sclerosis (ALS), like FTLD-TDP, is characterized by pathological TDP-43 inclusions. TMEM106B expression in the brain may be linked to mechanisms of disease in FTLD-TDP and risk alleles confer genetic susceptibility by increasing gene expression. TMEM106B can be showed as 31-55 kDa and 70-90 kDa (Glycosylated or Dimer) form in western blot test. (PMID: 27543298, 22895706, PMID: 23136129).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for TMEM106B (150-274aa) antibody 20995-1-APProtokoll herunterladen
IHC protocol for TMEM106B (150-274aa) antibody 20995-1-APProtokoll herunterladenl
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
human

Cell

Homotypic fibrillization of TMEM106B across diverse neurodegenerative diseases.

Authors - Andrew Chang
humanWB

J Neuroinflammation

Progranulin haploinsufficiency mediates cytoplasmic TDP-43 aggregation with lysosomal abnormalities in human microglia

Authors - Wonjae Sung
humanWB

J Neurosci

TMEM106B, the risk gene for frontotemporal dementia, is regulated by the microRNA-132/212 cluster and affects progranulin pathways.

Authors - Chen-Plotkin Alice S AS
humanWB

Alzheimers Res Ther

TMEM106B expression is reduced in Alzheimer's disease brains.

Authors - Jun-Ichi Satoh
mouseIF

Mol Brain

TMEM106B, a frontotemporal lobar dementia (FTLD) modifier, associates with FTD-3-linked CHMP2B, a complex of ESCRT-III.

Authors - Mi-Hee Jun
humanWB

PLoS One

Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer.

Authors - Kui Xiao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Q (Verified Customer) (05-08-2025)

It seems in WB, it works better with human sample than with mouse sample.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse brain lysate, human postmortem brain lysate
FH

Carolyn (Verified Customer) (02-18-2022)

good signal, low background

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human brain tissue extract