TMEM106B Monoklonaler Antikörper

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• KD/KO Validated

TMEM106B Monoklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

CloneNo.

5D1F8

Kat-Nr. : 60333-1-Ig

Synonyme

TMEM106B, transmembrane protein 106B

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Galerie der Validierungsdaten

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  WB analysis using 60333-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60333-1-Ig (TMEM106B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60333-1-Ig

  WB result of TMEM106B antibody (60333-1-Ig; 1:2000; room temperature for 1.5 hours) with wild-type and TMEM106B knockout HeLa cells.

• 

  WB analysis of HeLa using 60333-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60333-1-Ig (TMEM106B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60333-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60333-1-Ig (TMEM106B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 60333-1-Ig

  IP result of anti-TMEM106B(IP:60333-1-Ig, 5ug; Detection:60333-1-Ig 1:500) with HeLa cells lysate 1720 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen, A549-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

60333-1-Ig bindet in WB, IP, IHC, ELISA TMEM106B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TMEM106B fusion protein Ag21448
• Vollständiger Name: transmembrane protein 106B
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: ~40 kDa
• GenBank-Zugangsnummer: BC033901
• Gene symbol: TMEM106B
• Gene ID (NCBI): 54664
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMEM106B is a genetic risk factor for frontotemporal lobar degeneration with TDP-43 inclusions (FTLD-TDP). Amyotrophic lateral sclerosis (ALS), like FTLD-TDP, is characterized by pathological TDP-43 inclusions. TMEM106B expression in the brain may be linked to mechanisms of disease in FTLD-TDP and risk alleles confer genetic susceptibility by increasing gene expression.

Publikationen

Species	Application	Title
human	IHC	Cell; Homotypic fibrillization of TMEM106B across diverse neurodegenerative diseases.; Authors - Andrew Chang
human	WB	Brain; C-terminal TMEM106B fragments in human brain correlate with disease-associated TMEM106B haplotypes; Authors - Cristina T Vicente
human	WB	Pathol Res Pract; TESC acts as a prognostic factor and promotes epithelial-mesenchymal transition progression in esophageal squamous carcinoma; Authors - Yanxin Dong
human	IHC	Acta Neuropathol; Accumulation of TMEM106B C-terminal fragments in neurodegenerative disease and aging; Authors - Jolien Perneel
human	WB	Front Cell Neurosci; Loss of TMEM106B exacerbates C9ALS/FTD DPR pathology by disrupting autophagosome maturation; Authors - Claudia S Bauer ; KD Validated