TMEM119 Polyklonaler Antikörper

TMEM119 Polyklonal Antikörper für IHC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(22)

Rezensionen (2)

Kat-Nr. : 27585-1-AP

Synonyme

UNQ731/PRO1415, transmembrane protein 119, Osteoblast induction factor, OBIF

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  IHC staining of mouse brain using 27585-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27585-1-AP (TMEM119 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 27585-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TMEM119 antibody (27585-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 27585-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using TMEM119 antibody (27585-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HEK-293 using 27585-1-AP

  1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug TMEM119 Polyclonal antibody (27585-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe, Mauslebergewebe
• Erfolgreiche Detektion in FC (Intra): HEK-293-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 13 publications below
• FC: See 1 publications below

Produktinformation

27585-1-AP bindet in WB, IHC, IF-P, FC (Intra), ELISA TMEM119 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMEM119 fusion protein Ag26269
• Vollständiger Name: transmembrane protein 119
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: NM_181724
• Gene symbol: TMEM119
• Gene ID (NCBI): 338773
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMEM119 immunohistochemistry might provide a useful tool for investigating the biology and pathology of human microglia(PMID: 26250788). Microglia can be detected clearly using Catalog#27585-1-AP.

Publikationen

Species	Application	Title
human	IHC	Sci Adv; Single-cell analysis of human basal cell carcinoma reveals novel regulators of tumor growth and the tumor microenvironment.; Authors - Christian F Guerrero-Juarez
mouse,human	FC	Nat Commun; P-selectin axis plays a key role in microglia immunophenotype and glioblastoma progression.; Authors - Eilam Yeini
human	IF	Glia; Transmembrane protein 119 is neither a specific nor a reliable marker for microglia.; Authors - Elise Vankriekelsvenne
mouse	IF	Pharmacol Res; MSCs-extracellular vesicles attenuated neuroinflammation, synapse damage and microglial phagocytosis after hypoxia-ischemia injury by preventing osteopontin expression.; Authors - Danqing Xin
mouse	IF	Front Immunol; Didymin Suppresses Microglia Pyroptosis and Neuroinflammation Through the Asc/Caspase-1/GSDMD Pathway Following Experimental Intracerebral Hemorrhage.; Authors - Lingui Gu
human		Neurochem Res; BMP7 Attenuates Neuroinflammation after Spinal Cord Injury by Suppressing the Microglia Activation and Inducing Microglial Polarization Via the STAT3 Pathway; Authors - Xiaojin Wei

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marion (Verified Customer) (07-15-2025)

Immunohistofluorescence (PFA-fixed cryosections) image of TMEM119 stained murine brain. Blocking step : 10 % Donkey Serum + 1% BSA + 0.02% Triton as blocking agent for 2 hours at RT Primary antiboby incubation time : 1 night / 4°C Secondary antibody : AlexaFluor 568 (ab175470 ; Abcam) ; 1/200 This antibody does not seem to work under these conditions.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/200 (Also tested at 1/100; 1/400; 1/600 and 1/800)
• Cell Tissue Type: Mouse Brain

TMEM119 Antibody Immunofluorescence validation (1/200 (Also tested at 1/100; 1/400; 1/600 and 1/800) dilution) in Mouse Brain (Cat no:27585-1-AP)

FH

Hannes (Verified Customer) (07-27-2020)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHC-P image of anti-TMEM119 (27585-1-AP) stained murine and human brain. The tissue was paraffin-embedded, formalin fixed. The tissue was then incubated with EnVision+ Single Reagents (HRP. Rabbit), undiluted for 1 hour at room temperature.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Murine and human brain tissue

TMEM119 Antibody Immunohistochemistry validation (1:500 dilution) in Murine and human brain tissue (Cat no:27585-1-AP)