TMEM161A Polyklonaler Antikörper

TMEM161A Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24898-1-AP

Synonyme

AROS 29, TMEM161A, transmembrane protein 161A

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 24898-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24898-1-AP (TMEM161A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 24898-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24898-1-AP (TMEM161A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 24898-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 24898-1-AP (TMEM161A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 24898-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 24898-1-AP (TMEM161A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 24898-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24898-1-AP (TMEM161A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 24898-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24898-1-AP (TMEM161A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 24898-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24898-1-AP (TMEM161A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 24898-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24898-1-AP (TMEM161A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24898-1-AP bindet in WB, IHC,ELISA TMEM161A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMEM161A fusion protein Ag18974
• Vollständiger Name: transmembrane protein 161A
• Berechnetes Molekulargewicht: 479 aa, 54 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC005210
• Gene symbol: TMEM161A
• Gene ID (NCBI): 54929
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMEM161A also named as transmembrane protein 161A is a 479 amino-acid protein, which belongs to TMEM161 family. The molecular weight of TMEM161A is 56 kDa. TMEM161A is multi-pass membrane protein and localizes on the cell membrane. It may involve in the negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage and cellular response to oxidative stress.