TMEM59 Polyklonaler Antikörper

TMEM59 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 24134-1-AP

Synonyme

C1orf8, HSPC001, Liver membrane bound protein, TMEM59, transmembrane protein 59, UNQ169/PRO195

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  WB analysis of MCF-7 using 24134-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24134-1-AP (TMEM59 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 24134-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24134-1-AP (TMEM59 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 24134-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24134-1-AP (TMEM59 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 24134-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 24134-1-AP (TMEM59 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 24134-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 24134-1-AP (TMEM59 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 24134-1-AP

  Immunofluorescent analysis of HepG2 cells using 24134-1-AP (TMEM59 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

24134-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA TMEM59 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMEM59 fusion protein Ag21150
• Vollständiger Name: transmembrane protein 59
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: BC016374
• Gene symbol: TMEM59
• Gene ID (NCBI): 9528
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMEM59 is a regulator of autophagy in response to bacterial infection. Overexpression of this protein has been found to promote apoptosis in a glioma cell line. Alternative splicing of TMEM59 results in multiple transcript variants.

Publikationen

Species	Application	Title
human	WB	Mol Cell; An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress.; Authors - Julian Nüchel
human	WB	J Extracell Vesicles; A multi-omics approach identifies pancreatic cancer cell extracellular vesicles as mediators of the unfolded protein response in normal pancreatic epithelial cells.; Authors - Charles P Hinzman
	WB	Nat Commun; Progenitor translatome changes coordinated by Tsc1 increase perception of Wnt signals to end nephrogenesis.; Authors - Alison E Jarmas
bovine	WB,IP,CoIP	J Ethnopharmacol; In vivo and in vitro anti-inflammation of Rhapontici Radix extract on mastitis via TMEM59 and GPR161; Authors - Xiang Lv