TMIGD2/CD28H Rekombinanter Antikörper

TMIGD2/CD28H Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241807A5

Kat-Nr. : 98263-1-RR

Synonyme

TMIGD2/CD28, Immunoglobulin and proline-rich receptor 1, IGPR-1, IGPR1, CD28H

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  FC experiment of Transfected HEK-293T using 98263-1-RR

  1x10^6 HEK-293T cells or TMIGD2/CD28H transfected HEK-293T cells were stained with 0.25 ug Anti-Human TMIGD2/CD28H Rabbit Recombinant Antibody (98263-1-RR, Clone:241807A5) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Transfected HEK-293T using 98263-1-RR

  1x10^6 HEK-293T cells or TMIGD2/CD28H transfected HEK-293T cells were stained with 0.25 ug Anti-Human TMIGD2/CD28H Rabbit Recombinant Antibody (98263-1-RR, Clone: 241807A5) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 98263-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98263-1-RR against Human TMIGD2/CD28H were performed. The affinity constant is 171 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Transfected HEK-293T cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98263-1-RR bindet in FC TMIGD2/CD28H und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: transmembrane and immunoglobulin domain containing 2
• Berechnetes Molekulargewicht: 282 aa, 31 kDa
• GenBank-Zugangsnummer: BC015655
• Gene symbol: TMIGD2
• Gene ID (NCBI): 126259
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

Transmembrane and immunoglobulin domain-containing protein 2 (TMIGD2, also known as CD28H and IGPR1), plays a role in cell-cell interaction, migration, and angiogenesis. Through interaction with HHLA2, it costimulates T-cells in the context of TCR-mediated activation and enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade (PMID: 22419821; 23784006). TMIGD2 has been associated with several diseases, particularly in the context of cancer. It is aberrantly expressed by human AML cells and can be used to identify and enrich functional LSCs (PMID: 38167704).