TMPO/LAP2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TMPO/LAP2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Rezensionen (1)

Kat-Nr. : 14651-1-AP

Synonyme

CMD1T, LAP2, LEMD4, PRO0868, Splenin Thymopentin, thymopoietin, Thymopoietin isoform alpha, TMPO, TMPO/LAP2, TP, TP alpha, TP5, TPRP isoform alpha

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Galerie der Validierungsdaten

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  WB analysis using 14651-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14651-1-AP

  WB result of TMPO/LAP2 antibody (14651-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMPO/LAP2 transfected HeLa cells.

• 

  IP experiment of HeLa using 14651-1-AP

  IP Result of anti-LAP2 (IP:14651-1-AP, 4ug; Detection:14651-1-AP 1:500) with HeLa cells lysate 1600ug.

• 

  IHC staining of human breast cancer using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 14651-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14651-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMPO/LAP2 antibody (14651-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa cells using 14651-1-AP

  IF result of anti-TMPO (14651-1-AP) in Hela cell by Dr. Ralph Kehlenbach.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lebergewebe, humanes Leberkarzinomgewebe, Maushirngewebe, Mauslungengewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 3 publications below

Produktinformation

14651-1-AP bindet in WB, IP, IHC, IF, ELISA TMPO/LAP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMPO/LAP2 fusion protein Ag6247
• Vollständiger Name: thymopoietin
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 75 kDa, 51 kDa and 39 kDa
• GenBank-Zugangsnummer: BC053675
• Gene symbol: TMPO
• Gene ID (NCBI): 7112
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMPO resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. TMPO gene encodes 3 thymopoietins: alpha (75 kD), beta (51 kD), and gamma (39 kD).

Publikationen

Species	Application	Title
mouse		Nat Commun; The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus.; Authors - Ying Zhang
human	IF	J Cell Biol; A toolbox of anti-mouse and anti-rabbit IgG secondary nanobodies.; Authors - Tino Pleiner
human	WB	Sci Rep; MacroH2A1 associates with nuclear lamina and maintains chromatin architecture in mouse liver cells.; Authors - Yuhua Fu
bovine	WB	J Dairy Sci; Anti-inflammation of epicatechin mediated by TMEM35A and TMPO in bovine mammary epithelial cell line cells and mouse mammary gland.; Authors - Xiao Ma ; KO Validated
human	WB	World J Surg Oncol; Depletion of thymopoietin inhibits proliferation and induces cell cycle arrest/apoptosis in glioblastoma cells.; Authors - Lin Zhang ; KD Validated
mouse	IF	Theranostics; Progerin accumulation in nucleus pulposus cells impairs mitochondrial function and induces intervertebral disc degeneration and therapeutic effects of sulforaphane.; Authors - Xiaolong Xu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lauren (Verified Customer) (02-20-2020)

Tested fixes include -20'C Methanol for 10 mins, and both 2% and 4% Formaldehyde for 20 mins. We saw a good signal in all three fixes.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mIMCD3

TMPO/LAP2 Antibody Immunofluorescence, validation (1:100 dilution) in mIMCD3 (Cat no:14651-1-AP)