TNF beta Rekombinanter Antikörper

TNF beta Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

240393C3

Kat-Nr. : CL594-98056

Synonyme

LTA, 240393C3, LT alpha, Lymphotoxin alpha, Lymphotoxin-alpha

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  FC experiment of human PBMCs using CL594-98056

  1x10^6 untreated or anti-CD3/CD28 treated human PBMCs were intracellularly stained with 5 ul CoraLite®594 Anti-Human TNF beta Rabbit RecAb (CL594-98056, Clone: 240393C3). Cells were co-stained with FITC Plus Anti-Human CD3 (OKT3) (FITC-65133, Clone: OKT3). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): Anti-CD3/CD28 treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-98056 bindet in FC (Intra) TNF beta und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TNF beta fusion protein Eg0836
• Vollständiger Name: lymphotoxin alpha (TNF superfamily, member 1)
• Berechnetes Molekulargewicht: 205 aa, 22 kDa
• GenBank-Zugangsnummer: BC034729
• Gene symbol: TNF beta
• Gene ID (NCBI): 4049
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

TNF beta also known as lymphotoxin alpha (LTA), is a member of the tumor necrosis factor family, and is a cytokine produced by lymphocytes. TNF Beta is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. TNF Beta also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.