TNPO1 Polyklonaler Antikörper

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• KD/KO Validated

TNPO1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Rezensionen (4)

Kat-Nr. : 20679-1-AP

Synonyme

transportin 1, Transportin1, Transportin-1, Importin beta 2, Importin beta-2

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  WB analysis of HeLa using 20679-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20679-1-AP (Transportin-1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20679-1-AP

  WB result of TNPO1 antibody (20679-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-TNPO1 transfected HeLa cells.

• 

  WB analysis of mouse liver using 20679-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20679-1-AP (Transportin-1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 20679-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20679-1-AP (Transportin-1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20679-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20679-1-AP (Transportin-1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse lung using 20679-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20679-1-AP (Transportin-1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 20679-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TNPO1 antibody (20679-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, L02-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IHC: Mauslungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 3 publications below

Produktinformation

20679-1-AP bindet in WB, IHC, IF/ICC, ELISA TNPO1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: transportin 1
• Berechnetes Molekulargewicht: 102 kDa
• Beobachtetes Molekulargewicht: 95-102 kDa
• GenBank-Zugangsnummer: NM_002270
• Gene symbol: Transportin-1
• Gene ID (NCBI): 3842
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNPO1, also named as KPNB2, MIP1, TRN and MIP, belongs to the importin beta family. TNPO1 functions in nuclear protein import as nuclear transport receptor. It serves as receptor for nuclear localization signals (NLS) in cargo substrates. TNPO1 is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. TNPO1 is involved in nuclear import of M9-containing proteins. In vitro, it binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. It is involved in hnRNP A1/A2 nuclear export. TNPO1 mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. It binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, it mediates nuclear import of H2A, H2B, H3 and H4 histones, and SRP19. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. The antibody is specific to TNPO1.

Publikationen

Species	Application	Title
human	WB,IF	PLoS Genet; MxB inhibits long interspersed element type 1 retrotransposition.; Authors - Yu Huang
human	IF	Sci Rep; Nuclear RNA transcript levels modulate nucleocytoplasmic distribution of ALS/FTD-associated protein FUS.; Authors - Yueh-Lin Tsai
human	IF	Cell Rep; Antiviral Immune Response as a Trigger of FUS Proteinopathy in Amyotrophic Lateral Sclerosis.; Authors - Tatyana A Shelkovnikova
human	WB	Small; Effects of Puerarin-Loaded Tetrahedral Framework Nucleic Acids on Osteonecrosis of the Femoral Head; Authors - Yuxuan Zhao
human	WB	J Biol Chem; The nuclear transportation of CHRONO regulates the circadian rhythm; Authors - Qin Zhou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

SONAL (Verified Customer) (09-12-2025)

GOOD

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Beas 2 B Cells

FH

SONAL (Verified Customer) (09-12-2025)

GOOD

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Beas 2 B Cells

FH

SONAL (Verified Customer) (09-02-2025)

GOOD

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Beas 2 B Cells

FH

María (Verified Customer) (02-08-2022)

Works good for WB (1:1000). For IF, some background but works ok (1:100)

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: RPE-1/MSC