TOB1 Polyklonaler Antikörper

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• KD/KO Validated

TOB1 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 14915-1-AP

Synonyme

Transducer of erbB-2 1, Transducer of erbB 2 1, TOB, Protein Tob1, PIG49

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  WB analysis of HeLa using 14915-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14915-1-AP (TOB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14915-1-AP

  IP result of anti-TOB1 (IP:14915-1-AP, 4ug; Detection:14915-1-AP 1:1000) with HeLa cells lysate 1600ug.

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  IHC staining of human stomach cancer using 14915-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14915-1-AP (TOB1 antibody) at dilution of 1:100 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 14915-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14915-1-AP (TOB1 antibody) at dilution of 1:100 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

14915-1-AP bindet in WB, IHC, IF, IP, ELISA TOB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TOB1 fusion protein Ag6714
• Vollständiger Name: transducer of ERBB2, 1
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 38-45 kDa
• GenBank-Zugangsnummer: BC031406
• Gene symbol: TOB1
• Gene ID (NCBI): 10140
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TOB1, also known as APRO6 or TROB1, is a member of the transducer of erbB-2/B-cell translocation gene protein family. Members of this family are anti-proliferative factors that have the potential to regulate cell growth. The TOB1 protein has a large additional C-terminal region, which contains PAM2 motifs that mediate interactions with poly(A)-binding proteins. It is mainly located in the cell nucleus and will transfer between the cell nucleus and the cytoplasm. The more phosphorylation, the more obvious the cytoplasmic localization.The predicted MW of this protein is 38 kDa. Catalog#14915-1-AP recognises 38 and 45 kDa band, and the additional 45 kDa band may be due to phosphorylation.

Publikationen

Species	Application	Title
human	WB	Oncotarget; Screening key microRNAs for castration-resistant prostate cancer based on miRNA/mRNA functional synergistic network.; Authors - Jin Zhu
mouse	IHC	RNA Biol; NEDD4 triggers FOXA1 ubiquitination and promotes colon cancer progression under microRNA-340-5p suppression and ATF1 upregulation.; Authors - Meng Yue
human	WB	RNA Biol; Preferential translation of p53 target genes.; Authors - Miharu Hisaoka
human	WB	Am J Transl Res; Phosphorylation of TOB1 at T172 and S320 is critical for gastric cancer proliferation and progression.; Authors - Dong Wang
human	WB,IHC	Cancer Med; TOB1 suppresses proliferation in K-Ras wild-type pancreatic cancer.; Authors - Yuru Bai
mouse,human	WB,IF,IP	Commun Biol; TOB1 attenuates IRF3-directed antiviral responses by recruiting HDAC8 to specifically suppress IFN-β expression; Authors - Zhongxia Yu ; KO Validated