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  • Featured Product
  • KD/KO Validated

TOM70 Polyklonaler Antikörper

TOM70 Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Kat-Nr. : CL594-14528

Synonyme

FLJ90470, KIAA0719, KIAA0719, TOM70, TOM70, TOMM70A

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated TOM70 Polyclonal antibody (CL594-14528)

    IF Staining of HepG2 using CL594-14528

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 TOM70 antibody (CL594-14528) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated TOM70 Polyclonal antibody (CL594-14528)

    FC experiment of HepG2 using CL594-14528

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TOM70 (CL594-14528) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHepG2-Zellen
Erfolgreiche Detektion in FCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-14528 bindet in IF, FC (Intra) TOM70 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TOM70 fusion protein Ag6027
Vollständiger Name translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae)
Berechnetes Molekulargewicht 67 kDa
GenBank-ZugangsnummerBC052994
Gene symbol TOMM70A
Gene ID (NCBI) 9868
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TOMM70A (or TOM70) is a mitochondrial import receptor responsible for the import of cytosol synthesized precursor proteins into the mitochondria. Tom70 is a member of the tetratricopeptide repeat (TPR) co-chaperone family and contains a conserved TPR clamp domain for interaction with Hsc70 and Hsp90. Recently overexpression of TOM70 has been linked to chemoresistance to drugs in breast cancer cell lines. It is also believed that Tom70 is significantly associated with breast cancer relapse and may predict the recurrence of ovarian cancer.

Protokolle

Produktspezifische Protokolle
IF protocol for CL594 TOM70 antibody CL594-14528Protokoll herunterladen
FC protocol for CL594 TOM70 antibody CL594-14528Protokoll herunterladen
Standard-Protokolle
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