TOP1 Polyklonaler Antikörper

TOP1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

Kat-Nr. : 20705-1-AP

Synonyme

Top-I, TOPI, EC:5.6.2.1, DNA topoisomerase I, DNA topoisomerase 1

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  WB analysis using 20705-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20705-1-AP (TOP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 20705-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20705-1-AP (TOP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 20705-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20705-1-AP (TOP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 20705-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20705-1-AP (TOP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 20705-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20705-1-AP (TOP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 20705-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20705-1-AP (TOP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 20705-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20705-1-AP (TOP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 20705-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20705-1-AP (TOP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 20705-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20705-1-AP (TOP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 20705-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOP1 antibody (20705-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Zervixkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 15 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

20705-1-AP bindet in WB, IHC, IF/ICC, ELISA TOP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: topoisomerase (DNA) I
• Berechnetes Molekulargewicht: 91 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: NM_003286
• Gene symbol: TOP1
• Gene ID (NCBI): 7150
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TOP1 belongs to the eukaryotic type I topoisomerase family. The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. The antibody is specific to TOP1.

Publikationen

Species	Application	Title
human	WB,IF	Nat Commun; Topoisomerase 1 cleavage complex enables pattern recognition and inflammation during senescence.; Authors - Bo Zhao
human	WB, IF	Cell Chem Biol; Lipid-derived electrophiles mediate the effects of chemotherapeutic topoisomerase I poisons.; Authors - Amy Flor
human	WB	J Transl Med; Integrated multi-omics analyses reveal Jorunnamycin A as a novel suppressor for muscle-invasive bladder cancer by targeting FASN and TOP1; Authors - Ruijiao Chen
human	WB	Oncotarget; Genetic variants in microRNAs predict non-small cell lung cancer prognosis in Chinese female population in a prospective cohort study.; Authors - Xia Lingzi
human	WB	J Cell Mol Med; EGCG synergizes the therapeutic effect of irinotecan through enhanced DNA damage in human colorectal cancer cells.; Authors - Wenbing Wu
mouse	WB	iScience; Sensitization of ovarian tumor to immune checkpoint blockade by boosting senescence-associated secretory phenotype.; Authors - Xue Hao