TOP2A-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TOP2A-Specific Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Kat-Nr. : 20233-1-AP

Synonyme

DNA topoisomerase 2 alpha, TOP2, TOP2A, TOP2A-Specific, TP2A

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 20233-1-AP

  WB result of TOP2A-Specific antibody (20233-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2A-Specific transfected HepG2 cells.

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  WB analysis using 20233-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20233-1-AP (TOP2A-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20233-1-AP

  IP Result of anti-TOP2A-Specific (IP:20233-1-AP, 4ug; Detection:20233-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human spleen using 20233-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20233-1-AP (TOP2A-Specific antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 20233-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20233-1-AP (TOP2A-Specific antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 20233-1-AP

  Immunofluorescent analysis of HepG2 cells, using TOP2A antibody 20233-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, L02-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Produktinformation

20233-1-AP bindet in WB, IP, IHC, IF, ELISA TOP2A-Specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: topoisomerase (DNA) II alpha 170kDa
• Berechnetes Molekulargewicht: 174 kDa
• Beobachtetes Molekulargewicht: 170-177 kDa
• GenBank-Zugangsnummer: NM_001067
• Gene symbol: TOP2A
• Gene ID (NCBI): 7153
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.

Publikationen

Species	Application	Title
mouse	IF	Science; Chromosomes. A comprehensive Xist interactome reveals cohesin repulsion and an RNA-directed chromosome conformation.; Authors - Anand Minajigi
mouse	WB	Nat Commun; Loss of phosphatase CTDNEP1 potentiates aggressive medulloblastoma by triggering MYC amplification and genomic instability; Authors - Zaili Luo
mouse	IHC	Research (Wash D C); Magnetic Fields Affect Alcoholic Liver Disease by Liver Cell Oxidative Stress and Proliferation Regulation; Authors - Chao Song
human	WB	J Cell Biol; LRR1-mediated replisome disassembly promotes DNA replication by recycling replisome components.; Authors - Yilin Fan
human	WB	Oncotarget; TOP2Ahigh is the phenotype of recurrence and metastasis whereas TOP2Aneg cells represent cancer stem cells in prostate cancer.; Authors - Xuefeng Li ; KD Validated
human	WB	J Med Chem; Optimization of the Natural Product Calothrixin A to Discover Novel Dual Topoisomerase I and II Inhibitors with Improved Anticancer Activity.; Authors - Xiaohong Yang