TOP2A-Specific Polyklonaler Antikörper

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• KD/KO Validated

TOP2A-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(25)

Kat-Nr. : 20233-1-AP

Synonyme

TOP2A, DNA topoisomerase 2 alpha, DNA topoisomerase 2-alpha, EC:5.6.2.2, TOP II

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  WB analysis of HepG2 using 20233-1-AP

  WB result of TOP2A-Specific antibody (20233-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2A-Specific transfected HepG2 cells.

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  WB analysis using 20233-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20233-1-AP (TOP2A-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 20233-1-AP

  IP result of anti-TOP2A-Specific (IP:20233-1-AP, 4ug; Detection:20233-1-AP 1:8000) with L02 cells lysate 1040 ug.

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  IHC staining of human ovary cancer using 20233-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20233-1-AP (TOP2A-Specific antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 20233-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TOP2A-Specific antibody (20233-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, L02-Zellen
• Erfolgreiche IP: L02-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 15 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Produktinformation

20233-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA TOP2A-Specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: topoisomerase (DNA) II alpha 170kDa
• Berechnetes Molekulargewicht: 174 kDa
• Beobachtetes Molekulargewicht: 170-177 kDa
• GenBank-Zugangsnummer: NM_001067
• Gene symbol: TOP2A
• Gene ID (NCBI): 7153
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.

Publikationen

Species	Application	Title
mouse	IF	Science; Chromosomes. A comprehensive Xist interactome reveals cohesin repulsion and an RNA-directed chromosome conformation.; Authors - Anand Minajigi
mouse	WB	Nat Commun; Loss of phosphatase CTDNEP1 potentiates aggressive medulloblastoma by triggering MYC amplification and genomic instability; Authors - Zaili Luo
		Nat Commun; Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.; Authors - Hu Lei
mouse	IHC	Research (Wash D C); Magnetic Fields Affect Alcoholic Liver Disease by Liver Cell Oxidative Stress and Proliferation Regulation; Authors - Chao Song
human	WB	J Cell Biol; LRR1-mediated replisome disassembly promotes DNA replication by recycling replisome components.; Authors - Yilin Fan
human	WB	Oncotarget; TOP2Ahigh is the phenotype of recurrence and metastasis whereas TOP2Aneg cells represent cancer stem cells in prostate cancer.; Authors - Xuefeng Li ; KD Validated