TOPBP1 Polyklonaler Antikörper

TOPBP1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 23340-1-AP

Synonyme

KIAA0259, TOP2BP1, TOPBP1

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 23340-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23340-1-AP (TOPBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 23340-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23340-1-AP (TOPBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 23340-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23340-1-AP (TOPBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

23340-1-AP bindet in WB, IF, ELISA TOPBP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TOPBP1 fusion protein Ag18961
• Vollständiger Name: topoisomerase (DNA) II binding protein 1
• Berechnetes Molekulargewicht: 1522 aa, 171 kDa
• Beobachtetes Molekulargewicht: 171 kDa
• GenBank-Zugangsnummer: BC126209
• Gene symbol: TOPBP1
• Gene ID (NCBI): 11073
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human DNA topoisomerase II binding protein 1 (TopBP1) contains eight BRCT motifs that are found in proteins regulating the DNA damage response, transcription, and replication (1). In addition, TopBP1 shares sequence similarity with the fission yeast Rad4/Cut5 protein and the budding yeast DPB11 protein, both of which are required for DNA damage control and/or replication checkpoint control (2,3). Phosphorylation of TopBP1 occurs in response to DNA double-strand breaks and replication blocks (2). TopBP1 forms nuclear foci and localizes to the sites of DNA damage or the arrested replication forks (2,3). Downregulation of TopBP1 leads to reduced cell survival, probably due to increased apoptosis (2). TopBP1 functions as a transcriptional coactivator by enhancing the human papillomavirus (HPV) transcription/replication factor E2 (1). In addition, the HECT-domain ubiquitin ligase, hHYD, cooperates with TopBP1 in DNA damage response (4). TopBP1 specifically interacts with the C-terminal region of topoisomerase II beta, which suggests a supportive role for TopBP1 in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands (5). The gene encoding TopBP1 maps to chromosome 3q22.2 (5).

Publikationen

Species	Application	Title
mouse	IF	Biol Reprod; UHRF1 is indispensable for meiotic sex chromosome inactivation and interacts with the DNA damage response pathway in mice.; Authors - Mengneng Xiong