TPI1 Polyklonaler Antikörper

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• KD/KO Validated

TPI1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (4)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

33

Kat-Nr. : 10713-1-AP

Synonyme

TIM, TPI, TPI1, Triose phosphate isomerase, Triosephosphate isomerase, triosephosphate isomerase 1

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Galerie der Validierungsdaten

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  WB analysis using 10713-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10713-1-AP

  WB result of TPI1 antibody (10713-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPI1 transfected HEK-293 cells.

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  WB analysis of mouse liver using 10713-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10713-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10713-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10713-1-PBS

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10713-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10713-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 10713-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 10713-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 10713-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10713-1-AP (TPI1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10713-1-AP

  IP Result of anti-TPI1 (IP:10713-1-AP, 3ug; Detection:10713-1-AP 1:1000) with HEK-293 cells lysate 2500ug.

• 

  IHC staining of human colon cancer using 10713-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10713-1-AP (TPI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10713-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10713-1-AP (TPI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A431-Zellen, HepG2-Zellen, Mauslebergewebe, Mausnierengewebe, NIH/3T3-Zellen, Rattenlebergewebe, Rattennierengewebe, RAW 264.7-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 30 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

10713-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA TPI1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, drosophila, Hausschwein, Hefe, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TPI1 fusion protein Ag1175
• Vollständiger Name: triosephosphate isomerase 1
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC007086
• Gene symbol: TPI1
• Gene ID (NCBI): 7167
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TPI1(Triosephosphate isomerase) is also named as TIM, TPI and belongs to the triosephosphate isomerase family. It is a key enzyme in cell metabolism that controls the glycolytic flow and energy production through the interconversion of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde 3-phosphate (G3P)(PMID:19251756). Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). It can exsit as a homodimer(PMID:18562316).

Publikationen

Species	Application	Title
mouse	WB	Cell; The histone deacetylase Sirt6 regulates glucose homeostasis via Hif1alpha.; Authors - Zhong Lei L
		Nat Chem Biol; SF2312 is a natural phosphonate inhibitor of enolase.; Authors - Paul G Leonard
human	WB	Nat Commun; Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.; Authors - Xiao-Hui Wang
human	WB	Cell Res; cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.; Authors - Linchong Sun
human	WB	Nat Commun; Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.; Authors - Xiaoyu Ma
human,mouse	WB,IHC,IF	Brain; Amyloid-dependent triosephosphate isomerase nitrotyrosination induces glycation and tau fibrillation.; Authors - Guix Francesc X FX