TPTE Polyklonaler Antikörper

TPTE Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12982-1-AP

Synonyme

Cancer/testis antigen 44, CT44, PTEN2, TPTE, Tumor antigen BJ HCC 5

---

Galerie der Validierungsdaten

• 

  WB analysis of HepG2 using 12982-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12982-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12982-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12982-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 12982-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 12982-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 12982-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 12982-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12982-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12982-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 12982-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12982-1-AP (TPTE Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 12982-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12982-1-AP (TPTE Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of BxPC-3 using 12982-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 12982-1-AP (TPTE antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, BxPC-3-Zellen, HEK-293-Zellen, humanes Hodengewebe, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: BxPC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

12982-1-AP bindet in WB, IHC, IF, ELISA TPTE und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TPTE fusion protein Ag3606
• Vollständiger Name: transmembrane phosphatase with tensin homology
• Berechnetes Molekulargewicht: 62 kDa, 64 kDa
• Beobachtetes Molekulargewicht: 62 kDa, 64 kDa
• GenBank-Zugangsnummer: BC028719
• Gene symbol: TPTE
• Gene ID (NCBI): 7179
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TPTE(Transmembrane phosphatase with tensin homology) is also named as CT44, BJ-HCC-5 and belongs to the non receptor class of the protein-tyrosine phosphatase family. The gene encodes a predicted polypeptide of 551 amino acids with at least 2 potential transmembrane domains and a tyrosine phosphatase motif. TPTE may be involved in the signal transduction pathways of the endocrine or spermatogenic function of the testis(PMID:10598804). It is putatively involved in linking the cell membrane to cytoskeleton. This protein is very similar in sequence to PTEN through the phosphatase and C2 domains, but lacks a PDZ binding motif and an extensively phosphorylated C-terminal tail(PMID:17240336). It has 4 isoforms produced by alternative splicing.