TREX1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TREX1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Rezensionen (1)

Kat-Nr. : 24876-1-AP

Synonyme

DRN3, Deoxyribonuclease III, CRV, AGS5, 3'-5' exonuclease TREX1

---

• 

  WB analysis using 24876-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24876-1-AP (TREX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 24876-1-AP

  IP result of anti-TREX1 (IP:24876-1-AP, 4ug; Detection:24876-1-AP 1:300) with HeLa cells lysate 1200ug.

• 

  IF Staining of HeLa using 24876-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TREX1 antibody (24876-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Produktinformation

24876-1-AP bindet in WB, IF/ICC, IP, ELISA TREX1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TREX1 fusion protein Ag18325
• Vollständiger Name: three prime repair exonuclease 1
• Berechnetes Molekulargewicht: 369 aa, 39 kDa
• Beobachtetes Molekulargewicht: 32-39 kDa
• GenBank-Zugangsnummer: BC023630
• Gene symbol: TREX1
• Gene ID (NCBI): 11277
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TREX1 (three prime repair exonuclease 1), also known as trophoblast expressed 1, CRV, AGS1, AGS5, DRN3, HERNS or DNase III, is a member of the exonuclease superfamily and belongs to the TREX family. TREX1 may play a role in DNA repair. TREX1 is expressed in thymus, spleen, liver, brain, heart, small intestine and colon. Mutations or defects in the gene encoding TREX1 have been associated with a variety of diseases, including systemic lupus erythematosus, chilblain lupus (CHBL), Aicardi-Goutieres syndrome type 1 (AGS1) and type 5 (AGS5).

Publikationen

Species	Application	Title
		J Neuropathol Exp Neurol; Retinal Vasculopathy With Cerebral Leukodystrophy: Clinicopathologic Features of an Autopsied Patient With a Heterozygous TREX 1 Mutation.; Authors - Rie Saito
human	WB	bioRxiv; Pathogen-driven CRISPR screens identify TREX1 as a regulator of DNA self-sensing during influenza virus infection; Authors - Cason R King ; KO Validated
human	IF,WB	Cell Host Microbe; Pathogen-driven CRISPR screens identify TREX1 as a regulator of DNA self-sensing during influenza virus infection; Authors - Cason R King
human	WB	Cell Rep; Pharmacological inhibition of neddylation impairs long interspersed element 1 retrotransposition; Authors - Yan Li
bovine	WB	Int J Mol Sci; Inactivation of Myostatin Delays Senescence via TREX1-SASP in Bovine Skeletal Muscle Cells; Authors - Miaomiao Yang
human	WB	Front Immunol; Two rare mutations in homozygosity synergize to silence TREX1 in Aicardi-Goutières syndrome; Authors - Tamar Rubin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Chantal (Verified Customer) (10-04-2022)

reconignises the specific band, validated by KO of TREX1. A little bit of background. the signal is weak at 1:1000 dilution, better at 1:500. Works on human and mouse cell lines.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: MEFs, Hela, HEK293FT, RPE1

TREX1 Antibody Western Blot validation (1:500 dilution) in MEFs, Hela, HEK293FT, RPE1 (Cat no:24876-1-AP)