TRIB2 Polyklonaler Antikörper

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• KD/KO Validated

TRIB2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 15359-1-AP

Synonyme

C5FW, GS3955, TRB 2, TRB2, TRIB2, Tribbles homolog 2

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  WB analysis of HeLa using 15359-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15359-1-AP (TRIB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15359-1-AP

  WB result of TRIB2 antibody (15359-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIB2 transfected HeLa cells.

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  WB analysis of Jurkat using 15359-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15359-1-AP (TRIB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 15359-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 15359-1-AP (TRIB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15359-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15359-1-AP (TRIB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Hirngewebe, humanes Herzgewebe, humanes Nierengewebe, humanes Lebergewebe, humanes Lungengewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Milzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

15359-1-AP bindet in WB, IF, IHC, ELISA TRIB2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIB2 fusion protein Ag7605
• Vollständiger Name: tribbles homolog 2 (Drosophila)
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC002637
• Gene symbol: TRIB2
• Gene ID (NCBI): 28951
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIB2, also named as TRB2 , belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and Tribbles subfamily. TRIB2 interacts with MAPK kinases and regulates activation of MAP kinases. It does not display kinase activity. Antibodies against TRIB2 are present in sera from patients with autoimmune uveitis(uniprot). TRIB2 is enriched in hypocretin neurons. Sera from narcolepsy patients with cataplexy had higher Trib2-specific antibody titers compared with either normal controls or patients with idiopathic hypersomnia, multiple sclerosis, or other inflammatory neurological disorders.

Publikationen

Species	Application	Title
mouse,human	WB,IF	iScience; FUS-dependent microRNA deregulations identify TRIB2 as a druggable target for ALS motor neurons; Authors - Wan Yun Ho
mouse	WB,IF	Exp Mol Med; Trib2 regulates the pluripotency of embryonic stem cells and enhances reprogramming efficiency.; Authors - Eun Kyoung Do ; KD Validated