TRIM14 Polyklonaler Antikörper

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• KD/KO Validated

TRIM14 Polyklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-15742

Synonyme

Tripartite motif-containing protein 14, tripartite motif containing 14, TRIM, KIAA0129

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  IF Staining of HeLa using CL488-15742

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 TRIM14 antibody (CL488-15742) at dilution of 1:200.

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  FC experiment of HeLa using CL488-15742

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Trim14 Polyclonal Antibody (CL488-15742)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-15742 bindet in IF/ICC, FC (Intra) TRIM14 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM14 fusion protein Ag8158
• Vollständiger Name: tripartite motif-containing 14
• Berechnetes Molekulargewicht: 442 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC006333
• Gene symbol: TRIM14
• Gene ID (NCBI): 9830
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tripartite motif-containing 14(TRIM14) belongs to the TRIM protein family that contains 3 zinc-binding domains, a RING, a B-box type1 and a B-box type2 and plays key roles in cellular proliferation, differentiation, development, transformation, and immune response mechanisms . TRIM4 inhibits the transcriptional activity of SPI1 in a dose-dependent manner. It may participate in the early stages of differentiation of embryonic stem cells, leading to the formation of embryoid bodies.