TRIM24 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TRIM24 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(46)

Rezensionen (1)

Kat-Nr. : 14208-1-AP

Synonyme

TIF1-alpha, TIF1A, TIF1, RING-type E3 ubiquitin transferase TIF1-alpha, EC:2.3.2.27

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  WB analysis of HeLa using 14208-1-AP

  WB result of TRIM24 antibody (14208-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM24 transfected HeLa cells.

• 

  WB analysis using 14208-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14208-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BGC-823 using 14208-1-AP

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14208-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 Antibody) at dilution of 1:1500 incubated at 4 degree celsius over night.

• 

  WB analysis of L02 using 14208-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14208-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14208-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14208-1-AP

  IP result of anti-TRIM24 (IP:14208-1-AP, 4ug; Detection:14208-1-AP 1:6000) with HeLa cells lysate 1085 ug.

• 

  IHC staining of human prostate cancer using 14208-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14208-1-AP (TRIM24 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human prostate cancer using 14208-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14208-1-AP (TRIM24 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 14208-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRIM24 antibody (14208-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, BGC-823-Zellen, humanes Herzgewebe, L02-Zellen, MCF-7-Zellen, Maus-Skelettmuskelgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 13 publications below
• WB: See 35 publications below
• IHC: See 18 publications below
• IF: See 8 publications below
• IP: See 5 publications below
• CoIP: See 1 publications below
• ChIP: See 5 publications below
• RIP: See 1 publications below

Produktinformation

14208-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA TRIM24 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM24 fusion protein Ag5417
• Vollständiger Name: tripartite motif-containing 24
• Berechnetes Molekulargewicht: 117 kDa
• Beobachtetes Molekulargewicht: 117 kDa
• GenBank-Zugangsnummer: BC028689
• Gene symbol: TRIM24
• Gene ID (NCBI): 8805
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM24, also named as RNF82, TIF1 and TIF1A, interacts selectively in vitro with the AF2-activating domain of the estrogen receptors. Association with DNA-bound estrogen receptors, it requires the presence of estradiol. It is a regulation protein of P53. Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) wich is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue.

Publikationen

Species	Application	Title
human	IHC	Nature; TRIM24 links a non-canonical histone signature to breast cancer.; Authors - Tsai Wen-Wei WW
human	WB,CoIP	Mol Cell; p53 engages the cGAS/STING cytosolic DNA sensing pathway for tumor suppression; Authors - Monisankar Ghosh ; KD Validated
human	WB	Mol Cell; TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains.; Authors - Ai Kaiho-Soma
human	WB	Nat Chem Biol; Functional TRIM24 degrader via conjugation of ineffectual bromodomain and VHL ligands.; Authors - Lara N Gechijian
human	WB, IP, IHC	Nat Commun; TRIM24 is an oncogenic transcriptional co-activator of STAT3 in glioblastoma.; Authors - Deguan Lv
human	WB,IF	Nat Commun; Identification of proximal SUMO-dependent interactors using SUMO-ID.; Authors - Orhi Barroso-Gomila

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Parijat (Verified Customer) (09-20-2023)

Good antibody for Western

• Applications: Western Blot