TRIM29 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TRIM29 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 17542-1-AP

Synonyme

ATDC, TRIM29, tripartite motif containing 29

---

Galerie der Validierungsdaten

• 

  WB analysis using 17542-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17542-1-AP (TRIM29 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17542-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17542-1-AP (TRIM29 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 17542-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17542-1-AP (TRIM29 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 17542-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17542-1-AP (TRIM29 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 17542-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17542-1-AP (TRIM29 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 17542-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17542-1-AP (TRIM29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 17542-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17542-1-AP (TRIM29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 17542-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17542-1-AP (TRIM29 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 17542-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17542-1-AP (TRIM29 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 17542-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 17542-1-AP (TRIM29 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 17542-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 17542-1-AP (TRIM29 Antibody) at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, BxPC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe, humanes Hautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

17542-1-AP bindet in WB, IP, IHC, IF, ELISA TRIM29 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM29 fusion protein Ag11600
• Vollständiger Name: tripartite motif-containing 29
• Berechnetes Molekulargewicht: 588 aa, 66 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC017352
• Gene symbol: TRIM29
• Gene ID (NCBI): 23650
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM29(Tripartite motif-containing protein 29) is also named as ATDC( Ataxia telangiectasia group D-associated protein).ATDC protein physically interacted with the intermediate filament protein vimentin, a protein kinase C substrate and colocalizing protein, and with an inhibitor of protein kinase C, PKCI1(PMID:7644499). ATDC gene is highly expressed in samples of primary pancreatic cancer and PanIN lesions compared to normal pancreas and chronic pancreatitis and the highly expressed protein in human pancreatic cancer,enhances cancer cell proliferation and in vivo tumorigenicity by upregulation of the Wnt/b-Catenin signaling pathway(Wang, L. etc.2007).

Publikationen

Species	Application	Title
human,mouse	WB,IHC,IP	Mol Cancer; CircIL4R activates the PI3K/AKT signaling pathway via the miR-761/TRIM29/PHLPP1 axis and promotes proliferation and metastasis in colorectal cancer.; Authors - Tao Jiang ; KD Validated
human	WB	Oncogenesis; Galectin-9 promotes a suppressive microenvironment in human cancer by enhancing STING degradation.; Authors - Chuan-Xia Zhang
human	WB,IHC	PLoS One; Ataxia-Telangiectasia Group D Complementing Gene (ATDC) Promotes Lung Cancer Cell Proliferation by Activating NF-κB Pathway.; Authors - Tang Zhong-Ping ZP
		Cancer Sci; Role of deltaNp63(pos) CD44v(pos) cells in the development of N-nitroso-tris-chloroethylurea-induced peripheral-type mouse lung squamous cell carcinomas.; Authors - Shotaro Yamano
human	WB	Cancer Biol Ther; iTRAQ-based quantitative proteomic analysis of differentially expressed proteins in chemoresistant nasopharyngeal carcinoma.; Authors - Kun Wang
human	WB,IHC	Cancer Res Treat; TRIM29 Overexpression Promotes Proliferation and Survival of Bladder Cancer Cells Through NF-κB Signaling.; Authors - Shu-Tao Tan ; KD Validated