TRIM32 Polyklonaler Antikörper

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• KD/KO Validated

TRIM32 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 10326-1-AP

Synonyme

72 kDa Tat interacting protein, BBS11, HT2A, LGMD2H, TATIP, TRIM32, tripartite motif containing 32, Zinc finger protein HT2A

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 10326-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10326-1-AP (TRIM32 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 10326-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10326-1-AP (TRIM32 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 10326-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10326-1-AP (TRIM32 antibody) at dilution of 1:25 (under 10x lens).

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  IHC staining of human colon using 10326-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10326-1-AP (TRIM32 antibody) at dilution of 1:25 (under 40x lens).

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  IF Staining of mouse embryo using 10326-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 10326-1-AP (TRIM32 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of hTERT-RPE1 cells using 10326-1-AP

  IF result (cytoplasm stain) of anti-BBS11 (10326-1-AP; 1:50) with hTERT-RPE1 cell by Dr. Moshe Kim.

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  FC experiment of A431 using 10326-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human TRIM32 (10326-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: hTERT-RPE1-Zellen, Mausembryogewebe
• Erfolgreiche Detektion in FC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

10326-1-AP bindet in WB, IP, IHC, IF, FC, ELISA TRIM32 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRIM32 fusion protein Ag0401
• Vollständiger Name: tripartite motif-containing 32
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 65-72 kDa
• GenBank-Zugangsnummer: BC003154
• Gene symbol: TRIM32
• Gene ID (NCBI): 22954
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM32(Tripartite motif-containing protein 32) is a widely expressed ubiquitin ligase that is localized to the Z-line in skeletal muscle(PMID:19349376). It promotes PIAS4 ubiquitination and degradation, and by controlling PIAS4 stability, regulates UVB-induced keratinocyte apoptosis through induction of NFkappaB(PMID: 16816390).

Publikationen

Species	Application	Title
human	WB	Sci Adv; An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis.; Authors - Molly M Wilson
mouse,human	IHC	J Am Soc Nephrol; Proteome Analysis of Isolated Podocytes Reveals Stress Responses in Glomerular Sclerosis.; Authors - Sybille Koehler
mouse	WB	Hum Mol Genet; Deficiency of the E3 ubiquitin ligase TRIM32 in mice leads to a myopathy with a neurogenic component.; Authors - Kudryashova Elena E ; KO Validated
human,rat	WB,IF,IP	FASEB J; Ubiquitin ligase TRIM32 promotes dendrite arborization by mediating degradation of the epigenetic factor CDYL.; Authors - Lei Liu ; KD Validated
human	IF	J Cell Sci; TRIM32, but not its muscular dystrophy-associated mutant, positively regulates and is targeted to autophagic degradation by p62/SQSTM1.; Authors - Katrine Stange Overå
human	WB	iScience; A nonautophagic role of ATG5 in regulating cell growth by targeting c-Myc for proteasome-mediated degradation.; Authors - Sheng Li