TRIM62 Rekombinanter Antikörper

TRIM62 Rekombinant Antikörper für IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

230195G5

Kat-Nr. : 82892-1-RR

Synonyme

230195G5, E3 ubiquitin-protein ligase TRIM62, EC:2.3.2.27, tripartite motif containing 62, Tripartite motif-containing protein 62

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  IF Staining of U2OS using 82892-1-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TRIM62 antibody (82892-1-RR, Clone: 230195G5 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 82892-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human TRIM62 (82892-1-RR, Clone:230195G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.5 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82892-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82892-1-RR against Human TRIM62 were performed. The affinity constant is 90.8 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82892-1-RR bindet in IF/ICC, ELISA TRIM62 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TRIM62 fusion protein Ag7143
• Vollständiger Name: tripartite motif-containing 62
• Berechnetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC001222
• Gene symbol: TRIM62
• Gene ID (NCBI): 55223
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM62(Tripartite motif-containing protein 62) belongs to the TRIM/RBCC family. TRIM proteins are involved in a broad range of biological processes, including some that underlie genetic disorders, neurological disorders and cancers. TRIM62 is shown to promote the release of HIV from infected cells, and a TRIM62 protein that has a mutation in its RING domain was more active than the native protein in promoting HIV release(PMID:18836477).