TRPC6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TRPC6 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 18236-1-AP

Synonyme

FSGS2, Transient receptor protein 6, TRP 6, TRP6, TRPC6

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 18236-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:500.

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  WB analysis of mouse lung using 18236-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 18236-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 18236-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 18236-1-AP

  IP Result of anti-TRPC6 (IP:18236-1-AP, 4ug; Detection:18236-1-AP 1:300) with mouse lung tissue lysate 3600ug.

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  IHC staining of human placenta using 18236-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18236-1-AP (TRPC6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 18236-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18236-1-AP (TRPC6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 18236-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRPC6 antibody (18236-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of A549 using 18236-1-AP

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human TRPC6 (18236-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mouse brain, COLO 320-Zellen, Mauslungengewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Produktinformation

18236-1-AP bindet in WB, IP, IHC, IF, FC, ELISA TRPC6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRPC6 fusion protein Ag12976
• Vollständiger Name: transient receptor potential cation channel, subfamily C, member 6
• Berechnetes Molekulargewicht: 931 aa, 106 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC093658
• Gene symbol: TRPC6
• Gene ID (NCBI): 7225
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transient receptor potential canonical (TRPC) channels mediate the influx of different types of cations through the cell membrane and are involved in many functions of the organism (PMID: 28508319). The TRPC subfamily consists of seven members, TRPC1, TRPC2, TRPC3, TRPC4, TRPC5, TRPC6, TRPC7 (PMID: 32872338). TRPC6 is a Ca(2+)-permeable non-selective cation channel expressed in most human tissues. TRPC6 has been considered as potential therapeutic targets for malignant glioma treatment (PMID: 35489424).

Publikationen

Species	Application	Title
human	FC	Cell; Regenerative Metaplastic Clones in COPD Lung Drive Inflammation and Fibrosis.; Authors - Wei Rao
mouse	WB,IF	Biomaterials; Nongenetic optical modulation of neural stem cell proliferation and neuronal/glial differentiation.; Authors - Meitian Wang
human	IF	Proc Natl Acad Sci U S A; TRPC6 channel translocation into phagosomal membrane augments phagosomal function.; Authors - Vladimir Riazanski ; KO Validated
mouse	WB	Phytomedicine; Deciphering the pharmacological mechanisms of Rostellularia procumbens (L) Nees. Extract alleviates adriamycin-induced nephropathy in vivo and in vitro; Authors - Zhongzhu Ai
mouse	WB	Br J Pharmacol; Catalpol Alleviates Adriamycin-Induced Nephropathy by Activating the SIRT1 Signalling Pathway In Vivo and In Vitro.; Authors - Jiangnan Zhang
rat	WB	Lab Invest; Amelioration of circadian disruption and calcium-handling protein defects by choline alleviates cardiac remodeling in abdominal aorta coarctation rats.; Authors - Xi He

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