TRPV6 Polyklonaler Antikörper

TRPV6 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Kat-Nr. : 13411-1-AP

Synonyme

CATL, CaT1, CaT like, CaT L, Calcium transport protein 1

---

• 

  WB analysis of human placenta using 13411-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13411-1-AP (TRPV6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 13411-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13411-1-AP (TRPV6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13411-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13411-1-AP (TRPV6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13411-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13411-1-AP (TRPV6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 13411-1-AP

  IP result of anti-TRPV6 (IP:13411-1-AP, 4ug; Detection:13411-1-AP 1:300) with mouse kidney tissue lysate 4000ug.

• 

  IF Staining of HeLa using 13411-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRPV6 antibody (13411-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, Mausnierengewebe, PC-3-Zellen
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IF: See 5 publications below

Produktinformation

13411-1-AP bindet in WB, IF/ICC, IP, ELISA TRPV6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRPV6 fusion protein Ag4060
• Vollständiger Name: transient receptor potential cation channel, subfamily V, member 6
• Berechnetes Molekulargewicht: 725 aa, 83 kDa
• Beobachtetes Molekulargewicht: 83 kDa, 70 kDa
• GenBank-Zugangsnummer: BC034814
• Gene symbol: TRPV6
• Gene ID (NCBI): 55503
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRPV6, also named as CAT1 or ECaC2, is a member of the transient receptor potential (TRP) family of membrane proteins. Unlike most TRP channels, TRPV6 and its closest relative, TRPV5, are calcium-selective channels. TRPV6 is highly expressed in the proximal intestine, placenta and exocrine tissues (PMID: 12869611). It is probably involved in calcium absorption in various tissues, including calcium reabsorption in kidney. TRPV6 is overexpressed in some cancers and exhibits oncogenic potential.

Publikationen

Species	Application	Title
human	WB	J Mol Cell Cardiol; TRPV6 protects ER stress-induced apoptosis via ATF6α-TRPV6-JNK pathway in human embryonic stem cell-derived cardiomyocytes.; Authors - Zhichao Li
human	IF	iScience; A microengineered Brain-Chip to model neuroinflammation in humans; Authors - Iosif Pediaditakis
human	WB,IF	Mol Cancer Res; PMID: 19996302; Authors - Bolanz Katrin A KA
mouse	WB	Front Pharmacol; Aqueous Extract of Mori Folium Exerts Bone Protective Effect Through Regulation of Calcium and Redox Homeostasis via PTH/VDR/CaBP and AGEs/RAGE/Nox4/NF-κB Signaling in Diabetic Rats.; Authors - Chenyue Liu
human	WB	J Gastroenterol Hepatol; Vitamin D receptor is overexpressed in the duodenum of patients with irritable bowel syndrome.; Authors - Ko Miura
Mouse	WB	Ann Transl Med; Rosavin suppresses osteoclastogenesis in vivo and in vitro by blocking the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways.; Authors - Wenhao Zhang