TRUB1 Polyklonaler Antikörper

TRUB1 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 12520-1-AP

Synonyme

EC:5.4.99.-, EC:5.4.99.25, Pseudouridylate synthase TRUB1, Psi55 synthase TRUB1, PUS4

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  WB analysis using 12520-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12520-1-AP (TRUB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 12520-1-AP

  IP result of anti-TRUB1 (IP:12520-1-AP, 4ug; Detection:12520-1-AP 1:300) with mouse liver tissue lysate 6400ug.

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  IHC staining of human kidney using 12520-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12520-1-AP (TRUB1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 12520-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12520-1-AP (TRUB1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse testis using 12520-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12520-1-AP (TRUB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 12520-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12520-1-AP (TRUB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HepG2-Zellen
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Produktinformation

12520-1-AP bindet in WB, IHC, IF, IP, ELISA TRUB1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRUB1 fusion protein Ag3213
• Vollständiger Name: TruB pseudouridine (psi) synthase homolog 1 (E. coli)
• Berechnetes Molekulargewicht: 349 aa, 37 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC030601
• Gene symbol: TRUB1
• Gene ID (NCBI): 142940
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRUB1 gene encodes a 349-amino acid product, with a VFAVHKPKGPTSA box in positions 71-83 corresponding to motif I of the TruB family (probably involved in conserving protein structure). This protein may be responsible for synthesis of pseudouridine from uracil in transfer RNAs.

Publikationen

Species	Application	Title
human	WB	Nat Methods; Absolute quantitative and base-resolution sequencing reveals comprehensive landscape of pseudouridine across the human transcriptome; Authors - Haiqi Xu
human	IF	Cell Syst; Probing enzyme-dependent pseudouridylation using direct RNA sequencing to assess epitranscriptome plasticity in a neuronal cell line; Authors - Oleksandra Fanari
human	WB	Cancer Discov; ALKBH1 drives tumorigenesis and drug resistance via tRNA decoding reprogramming and codon-biased translation; Authors - Chao Shen
human	IF	bioRxiv; Probing enzyme-dependent pseudouridylation using direct RNA sequencing to assess neuronal epitranscriptome plasticity; Authors - Oleksandra Fanari
human	WB	Nucleic Acids Res; A bisulfite-assisted and ligation-based qPCR amplification technology for locus-specific pseudouridine detection at base resolution; Authors - Xin Fang ; KD Validated