TSC22D2 Polyklonaler Antikörper

TSC22D2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 25418-1-AP

Synonyme

TSC22 domain family protein 2, TILZ4c, TILZ4b, TILZ4a, TILZ4

---

• 

  WB analysis using 25418-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25418-1-AP (TSC22D2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 25418-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25418-1-AP (TSC22D2 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of MDA-MB-453s using 25418-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 25418-1-AP (TSC22D2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 25418-1-AP

  IP result of anti-TSC22D2 (IP:25418-1-AP, 5ug; Detection:25418-1-AP 1:300) with HepG2 cells lysate 2500ug.

• 

  IHC staining of human kidney using 25418-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 25418-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 25418-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 25418-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 25418-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TSC22D2 antibody (25418-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, MDA-MB-453s-Zellen, SH-SY5Y-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

25418-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA TSC22D2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TSC22D2 fusion protein Ag22294
• Vollständiger Name: TSC22 domain family, member 2
• Berechnetes Molekulargewicht: 780 aa, 79 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC044643
• Gene symbol: TSC22D2
• Gene ID (NCBI): 9819
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSC22D2 (transforming growth factor β-stimulated clone 22 domain family, member 2) is a member of the TSC-22 domain family comprising putative transcription factors that are characterized by a carboxy-terminal leucine zipper and an adjacent TSC-box. TSC22D2 is expressed at low levels in CRC and that TSC22D2 overexpression significantly inhibited the growth rate of cancer cells (PMID: 27573352).

Publikationen

Species	Application	Title
human	WB,IHC	BMC Cancer; A novel cuproptosis-related gene model predicts outcomes and treatment responses in pancreatic adenocarcinoma; Authors - Qixian Liu