TSP50 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TSP50 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 12574-1-AP

Synonyme

Cancer/testis antigen 20, CT20, PRSS50, Serine protease 50, testes protease 50, TSP50

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 12574-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12574-1-AP (TSP50 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12574-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12574-1-AP (TSP50 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12574-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12574-1-AP (TSP50 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 12574-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12574-1-AP (TSP50 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12574-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12574-1-AP (TSP50 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12574-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12574-1-AP (TSP50 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 12574-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12574-1-AP (TSP50 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 12574-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12574-1-AP (TSP50 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 12574-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12574-1-AP (TSP50 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 12574-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12574-1-AP (TSP50 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 12574-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12574-1-AP (TSP50 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 12574-1-AP

  Immunofluorescent analysis of MCF-7 cells using 12574-1-AP (TSP50 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, HEK-293-Zellen, humanes Hodengewebe, Maushodengewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Hodengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below

Produktinformation

12574-1-AP bindet in WB, IHC, IF,ELISA TSP50 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TSP50 fusion protein Ag3247
• Vollständiger Name: testes-specific protease 50
• Berechnetes Molekulargewicht: 385 aa, 43 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC033016
• Gene symbol: TSP50
• Gene ID (NCBI): 29122
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSP50 (Testis-specific protease-like protein 50) is also named as PRSS50, CT20 and belongs to the peptidase S1 family. It can enhance the expression of NFKB target genes involved in cell proliferation, and promotes cell proliferation, at least partially, through activation of the NFKB signalling pathway(PMID:21385156). There are two protein bands of 37 kDa and 43 kDa to be detected in human testes that the the difference is most likely caused by posttranslational proteolytic cleavage(PMID:11782390). The full length protein has a signal peptide with 39 amino acids and two glycosylation sites.

Publikationen

Species	Application	Title
human	WB,IHC	BMC Cancer; Testes-specific protease 50 (TSP50) promotes invasion and metastasis by inducing EMT in gastric cancer.; Authors - Qing-Hua Cao ; KD Validated
		Oncol Rep; Lentivirus-mediated knockdown of TSP50 suppresses the growth of non-small cell lung cancer cells via G0/G1 phase arrest.; Authors - Wen-Liang Qiao
human	WB,IHC	Gastroenterol Res Pract; Overexpression of Testes-Specific Protease 50 (TSP50) Predicts Poor Prognosis in Patients with Gastric Cancer.; Authors - Fang Liu
human	IHC	Oncol Lett; Testes-specific protease 50 as an independent risk factor for poor prognosis in patients with non-small cell lung cancer.; Authors - Wen-Liang Qiao